Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Subviral Agents01:29

Subviral Agents

Subviral agents are infectious entities that resemble viruses but lack one or more viral components, such as a capsid or essential replication machinery. These agents include viroids, prions, and satellites, each possessing distinct structural and functional characteristics that influence their mode of infection and replication.Viroids are the simplest subviral agents, consisting of circular, single-stranded RNA molecules without a protein coat. They exclusively infect plants, relying entirely...
Viruses with RNA Genomes01:29

Viruses with RNA Genomes

RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
Viruses of Archaea01:29

Viruses of Archaea

Archaeal viruses play a crucial role in the ecosystems of extremophilic archaea, particularly those belonging to the phyla Euryarchaeota and Crenarchaeota. By shaping host evolution and facilitating gene transfer, these viruses influence microbial communities and contribute to genetic diversity in extreme environments. The archaea they infect thrive in acidic hot springs and hydrothermal vents characterized by high temperatures and low pH. Archaeal viruses exhibit remarkable structural...
IP3/DAG Signaling Pathway01:11

IP3/DAG Signaling Pathway

Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and produces two-second...
Arboviral Encephalitis01:25

Arboviral Encephalitis

Arboviral encephalitis refers to brain inflammation caused by arthropod-borne viruses, particularly those transmitted through mosquito vectors. Among these, West Nile virus (WNV), a member of the Flaviviridae family, is a significant public health concern. WNV is an enveloped, positive-sense, single-stranded RNA virus. Human infection typically begins when an infected mosquito introduces the virus into the dermis during feeding. The primary transmission cycle involves birds as amplifying hosts...
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparative transcriptomics of biopesticide-treated olives subjected to Xylella infection.

BMC plant biology·2026
Same author

Life cycle assessment of biopesticides and chemical pesticides for managing Xylella fastidiosa in olive trees.

Journal of environmental management·2026
Same author

The impact of assisted reproductive technology on the neonatal prognosis of newborns < 29 weeks gestational age: a retrospective case-control study.

Journal of gynecology obstetrics and human reproduction·2026
Same author

New viral sequences and endogenous viral elements (EVE) in world-wide populations of Trioza erytreae, the African citrus psyllid.

Virus research·2026
Same author

Effects of biopesticides on the progression of Olive Quick Decline Symptoms caused by <i>Xylella fastidiosa</i> subsp. <i>pauca</i>.

Plant disease·2026
Same author

Barriers students with epilepsy and their caregivers face regarding school participation and seizure management within school.

Epilepsy & behavior : E&B·2026
Same journal

A comparative genomic approach to identify determinants of meropenem resistance in <i>Klebsiella pneumoniae</i> using pan-genome-wide association analysis.

Frontiers in microbiology·2026
Same journal

Phthalazine-based quaternary ammonium salts: synthesis, biological evaluation and membrane-targeting mechanism against <i>Staphylococcus aureus</i>.

Frontiers in microbiology·2026
Same journal

Linking wet-lab and genomic approaches for reliable detection of carbapenemase-producing <i>Klebsiella pneumoniae</i> in wastewater.

Frontiers in microbiology·2026
Same journal

Synergistic modulation of the gut microbiome-liver-host metabolome axis associates with the therapeutic efficacy of Danlou tablet against metabolic syndrome.

Frontiers in microbiology·2026
Same journal

Microbial-enzymatic coupling drives nitrogen stabilization during static pile composting of rice straw amended with contrasting nitrogen sources.

Frontiers in microbiology·2026
Same journal

Editorial: Probiotics and bioactive agents in modulating harmful oral biofilms.

Frontiers in microbiology·2026
See all related articles

Related Experiment Video

Updated: May 12, 2026

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
10:40

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Published on: December 22, 2017

Grapevine leafroll-associated virus 3.

Hans J Maree1, Rodrigo P P Almeida, Rachelle Bester

  • 1Department of Genetics, Stellenbosch University Stellenbosch, South Africa ; Biotechnology Platform, Agricultural Research Council Stellenbosch, South Africa.

Frontiers in Microbiology
|April 19, 2013
PubMed
Summary
This summary is machine-generated.

Grapevine leafroll-associated virus 3 (GLRaV-3) causes significant economic losses in vineyards worldwide. Recent genomic studies reveal genetic variants, improving diagnostics and management strategies for this important grapevine virus.

Keywords:
ClosteroviridaeGLRaV-3ampelovirusgenetic variantsgrapevine leafroll disease

More Related Videos

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
08:56

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

Published on: July 27, 2018

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens
10:19

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

Published on: February 3, 2020

Related Experiment Videos

Last Updated: May 12, 2026

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
10:40

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Published on: December 22, 2017

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
08:56

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

Published on: July 27, 2018

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens
10:19

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

Published on: February 3, 2020

Area of Science:

  • Plant Pathology
  • Virology
  • Genomics

Background:

  • Grapevine leafroll disease (GLD) causes substantial economic losses globally.
  • Grapevine leafroll-associated virus 3 (GLRaV-3) is a primary causal agent of GLD.
  • Effective management requires understanding GLRaV-3's genetic diversity and infection mechanisms.

Purpose of the Study:

  • To provide an overview of GLRaV-3, focusing on recent genomic characterization.
  • To discuss the classification of GLRaV-3 genetic variants.
  • To highlight advancements in diagnostic techniques for GLRaV-3.

Main Methods:

  • Genome sequencing and annotation of GLRaV-3 isolates.
  • Analysis of subgenomic RNAs (sgRNAs) to understand replication.
  • Comparison of molecular diagnostic techniques with serological assays.
  • Application of next-generation sequencing (NGS) for viral interaction studies.

Main Results:

  • Full genome sequencing revealed diverse genetic variants of GLRaV-3.
  • sgRNA characterization provided insights into viral replication and gene function.
  • Nucleotide sequence-based diagnostics are more sensitive than ELISA.
  • NGS offers comprehensive data on viral complexes and variants.

Conclusions:

  • Genomic insights are crucial for classifying GLRaV-3 variants and developing management strategies.
  • Advanced molecular diagnostics, particularly NGS, enhance virus detection and understanding of disease complexes.
  • Reliable virus screening is essential for grapevine certification and GLD control.