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Updated: May 12, 2026
Detection of Enterohemorrhagic Escherichia Coli Colonization in Murine Host by Non-invasive In Vivo Bioluminescence System
Published on: April 9, 2018
GFP-tagged E. coli shows bacterial distribution in mouse organs: pathogen tracking using fluorescence signal
Pil-Gu Park1, Min-Hee Cho, Gi-Eun Rhie
1Division of High-Risk Pathogen Research, Center for Infectious Diseases, Korea National Institute of Health, Cheongwon, Korea.
Purpose:
In vaccine efficacy evaluation, visualization of pathogens in whole organism at each time point would be able to reduce the consuming animals and provide the in vivo information within consistent background with identical organism.
Materials And Methods:
Using IVIS spectrum whole live-animal imaging system, fluorescent intensity was optimized and visualized proportionately by concentrating Escherichia coli MC1061 strain which expresses GFP (E. coli-GFP) in BALB/C mice after injection.
Results:
Local distribution of disseminated E. coli-GFP was traced in each organ by fluorescence. Detached organ showed more obvious fluorescent signal, and intestine showed strongest fluorescent signal.
Conclusion:
This in vivo imaging method using GFP-tagged pathogen strain suggest quantified infected pathogens by fluorescence intensity in whole animals can provide the information about the localization and distribution after infection.
