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Published on: July 28, 2010
[Study on association between ERCC2/XPD single nucleotide polymorphisms and DNA adducts damage induced by B[a]P in
1China Medical University, Public Health Institute, Toxicology Department, Shenyang 110001, China. cfliuyh@126.com
Objective:
To evaluate the association between DNA damage repair capacity induced by environment carcinogen benzo[a] pyrene (B [a] P) and ERCC2/XPD single nucleotide polymorphisms(SNP).
Methods:
8 ml peripheral bloods of 282 healthy ethnic Han people from Liao-ning province were collected, isolated lymphocytes and extracted DNA. The genotypes of ERCC2/XPD Lys751 Gln (rs13181), Asp312Asn (rs1799793), Arg156Arg (rs238406) were detected by Taqman real time PCR; BPDE-DNA adduct in vitro induced by B[a]P and S9 mixture in lymphocyte were detected by high performance liquid chromatography (HPLC).
Results:
The BPDE-DNA adduct levels of ERCC2/XPD Arg156Arg AA genotype were significantly higher than CC genotype. Compared with < or = 30 years, people at age of 50 - 70 years and > or = 70 years have higher BPDE-DNA adduct level (P < 0.05). Multiple covariates analysis showed SNP of ERCC2/XPD Arg156Arg (rs238406) and age have been related to BPDE-DNA adduct levels closely among all covariates (P < 0.05).
Conclusion:
ERCC2/XPD Arg156Arg rs238406 polymorphisms may be associated with DNA repair capacity in excising BPDE-DNA adduct and A allele may increase the risks of cancer susceptibility.
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