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Mailuoning prevents high-glucose-mediated human umbilical vein endothelial cells apoptosis
1Department of Ophthalmonogy, Jiangsu Province Hospital on Integration of Chinese and Western Medicine, Nanjing 210028, China.
Objective:
To investigated the role of Mailuoning in the prevention of high-glucose-mediated cell apoptosis in human umbilical vein endothelial cells (HUVEC) and the mechanisms involved.
Methods:
MTT assay was used to investigate cell viability, western blot was used to investigate protein expression, and flow cytometric detection technology was used to detect cell apoptosis.
Results:
Exposure of HUVEC to high glucose (50 mM) significantly suppressed cell viability and increased cell apoptosis compared with normal glucose (11 mM) (all P < 0.05). However, Mailuoning prevented high-glucose-induced HUVEC apoptosis in dose-dependent manner. Further studies indicated that Mailuoning suppressed high-glucose-induced p38 mitogen-activated protein kinase phosphorylation, but had no effect on extracellular signal-regulated kinase 1/2 and Akt phosphorylation.
Conclusion:
Mailuoning can prevent high-glucose-induced HUVEC apoptosis by suppressing p38 activation.
