Related Experiment Video
Updated: May 12, 2026

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
Published on: April 11, 2016
Studies on structural and physical characteristics of a novel exopolysaccharide from Pseudozyma sp. NII 08165
Kuttuvan Valappil Sajna1, Rajeev K Sukumaran, Lalitha Devi Gottumukkala
1Biotechnology Division, CSIR - National Institute for Interdisciplinary Science and Technology, Trivandrum 695019, India.
Abstract:
The aim of this work was to study the production of exopolysaccharide (EPS) from a novel ustilaginomycetes yeast strain Pseudozyma sp. NII 08165. The culture produced 3.5g/l EPS on fourth day of fermentation in a glucose-based medium. The structural characterization revealed that the EPS was a polymer of glucose, galactose and mannose in the ratio of 2.4:5.0:2.6 with a molecular weight of 1.7MDa. The pseudoplastic behaviour of aqueous EPS with a thermal stability up to 220°C indicated its potential utility as a thickening or gelling agent in food industry. SEM studies of the EPS showed that it had compact film-like structure, which could make it a useful in preparing plasticized films. The AFM studies showed that EPS had spike-shaped microstructure. Physical properties of the exopolysaccharide determined further indicated its possible potential in different industrial applications.
Related Concept Videos
Biosynthesis of Polysaccharides
Formation of Lipopolysaccharides
Cellulose and Pectic Polysaccharides
As a cell matures, its cell wall specializes according to its type. For example, the parenchyma cells of...

