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Nanosponge Tunability in Size and Crosslinking Density
Published on: August 4, 2017
Solid phase extraction as a cleanup step before microextraction of diclofenac and mefenamic acid using nanostructured
Fatemeh Rezaei1, Yadollah Yamini, Morteza Moradi
1Department of Chemistry, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Iran.
Talanta
|April 20, 2013
Summary
A novel solid-phase extraction combined with supramolecular solvents (SUPRAS) method efficiently extracts diclofenac and mefenamic acid from complex samples. This approach offers high enrichment factors for accurate drug analysis in urine and water.
Area of Science:
- Analytical Chemistry
- Environmental Chemistry
Background:
- Diclofenac (DIC) and mefenamic acid (MEF) are common pharmaceuticals found in environmental and biological samples.
- Matrix effects in urine and water samples can interfere with accurate drug quantification.
Purpose of the Study:
- To develop and validate a new pretreatment method for extracting DIC and MEF from urine and water.
- To combine solid-phase extraction (SPE) with supramolecular solvent formation (SUPRASF) for enhanced analyte recovery.
Main Methods:
- Solid-phase extraction (SPE) for initial analyte isolation from sample matrices.
- Supramolecular solvent formation (SUPRASF) using decanoic acid (DeA) in tetrahydrofuran (THF) with water as a coacervating agent.
- High-performance liquid chromatography with UV detection (HPLC-UV) for final analyte separation and quantification.
Main Results:
- High enrichment factors achieved: 431 for MEF and 489 for DIC.
- Linear calibration curves with excellent coefficients of determination (r² from 0.996 to 0.999).
- Successful application to real urine and water samples with high relative recoveries (90.4–103.8%).
Conclusions:
- The SPE-SUPRASF method is effective for the simultaneous extraction and determination of DIC and MEF.
- This innovative technique minimizes matrix effects and enhances analytical sensitivity.
- The method is suitable for routine analysis of these pharmaceuticals in complex biological and environmental matrices.
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