Stump-like mathematical model and computer simulation on dynamic separation of capillary zone electrophoresis with...

In this paper, a novel mathematical model of stump-like peak was constructed for alternative computer simulation of capillary zone electrophoresis (CZE) by using Haarhoff-Van der Linde (HVL) function. Unlike a classical model of Gaussian peak, the developed model contains both Gaussian and plateau concentration distributions of analytes. Based on the model, the relevant computer software was developed and implemented in Borland Delphi 7 environment. The relevant results revealed that (i) the software could freely simulate the plateau and Gaussian distribution peaks; (ii) the simulator could simulate a dynamic process of CZE properly; and (iii) the program could display the final electropherogram of numerous analytes in CZE. We further conducted the relevant experiments and compared them with the simulations. The comparisons demonstrated the high agreement between the simulation results and the experiments as well as those cited from references. In addition, the software could calculate effective mobility, diffusion coefficient and concentration of analytes based on the physico-chemical parameters input by users. The developed model and software have evident significance for understanding of electrophoretic separation, conditional optimization and basic computation on physico-chemical parameters of analytes in CZE.