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Improving the understanding of oligonucleotide-nanoparticle conjugates using DNA-binding fluorophores

Luca Guerrini1, Lee Barrett, Jennifer A Dougan

  • 1Centre for Molecular Nanometrology, Department of Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow G1 1YL, UK. luca.guerrini@ctqc.org

Nanoscale
|April 20, 2013
PubMed

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Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...