Related Experiment Video
Updated: May 12, 2026

Chromatin Immunoprecipitation (ChIP) using Drosophila tissue
Published on: March 23, 2012
Comment on "Drosophila dosage compensation involves enhanced Pol II recruitment to male X-linked promoters"
F Ferrari1, Y L Jung, P V Kharchenko
1Center for Biomedical Informatics, Harvard Medical School, Boston, MA 02115, USA.
Abstract:
Conrad et al. (Reports, 10 August 2012, p. 742) reported a doubling of RNA polymerase II (Pol II) occupancy at X-linked promoters to support 5' recruitment as the key mechanism for dosage compensation in Drosophila. However, they employed an erroneous data-processing step, overestimating Pol II differences. Reanalysis of the data fails to support the authors' model for dosage compensation.
Related Concept Videos
Dosage Compensation
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will have...
The Ratio of X Chromosome to Autosomes
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female Drosophila...
Position-effect Variegation
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Cis-regulatory Sequences

