Related Experiment Video

Updated: May 12, 2026

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

A long non-coding RNA, PTCSC3, as a tumor suppressor and a target of miRNAs in thyroid cancer cells

Min Fan1, Xinying Li, Wei Jiang

  • 1Department of General Surgery, Xiangya Hospital, Central South University, Changsha 410008; ; Department of Geriatrics, Xiangya Second Hospital, Central South University, Changsha 410011, P.R. China.

Experimental and Therapeutic Medicine
|April 20, 2013
PubMed

Insights

Newly identified PTCSC3 (Papillary thyroid carcinoma susceptibility candidate 3) acts as a tumor suppressor in thyroid cancer. Its downregulation inhibits cancer cell growth, arrests the cell cycle, and promotes apoptosis, potentially via interaction with miR-574-5p.

Area of Science:

  • Molecular Biology
  • Genetics
  • Oncology

Background:

  • PTCSC3 is a novel, thyroid-specific non-coding RNA.
  • Significant downregulation of PTCSC3 in thyroid cancers suggests a tumor-suppressive role.
  • Understanding PTCSC3's function is crucial for thyroid cancer research.

Purpose of the Study:

  • To investigate the impact of PTCSC3 on thyroid cancer cell biological features.
  • To explore PTCSC3's function as a competing endogenous RNA (ceRNA) interacting with microRNAs (miRNAs).

Main Methods:

  • Transfection of PTCSC3 constructs into thyroid cancer cell lines (BCPAP, FTC133, 8505C).
  • Assessment of cell growth, cell cycle, and apoptosis using MTT assay and flow cytometry.
  • In silico analysis to predict miRNA binding sites and RT-PCR to confirm miRNA interactions.

Main Results:

  • PTCSC3 transfection significantly inhibited growth, induced cell cycle arrest, and increased apoptosis in all tested thyroid cancer cell lines.
  • Potential interactions between PTCSC3 and top 20 miRNAs were identified.
  • An inverse correlation between PTCSC3 and miR-574-5p was confirmed in vitro.

Conclusions:

  • PTCSC3 functions as a tumor suppressor in thyroid cancer.
  • PTCSC3 acts as a competing endogenous RNA for miR-574-5p, influencing thyroid cancer progression.
Keywords:
miR-574-5ppapillary thryoid susceptibility candidate 3thyroid cancer

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...