Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inflammation01:38

Inflammation

Overview
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR activation may...
Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs01:25

Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs

Asthma is a chronic respiratory condition for which new therapeutic avenues, including anti-inflammatory drugs like mast cell stabilizers and anti-IgE treatments, continue to be developed.
Mast cell stabilizers, such as cromolyn (also known as sodium cromoglycate) and nedocromil (Tilade), are effective drugs in asthma management. These stabilizers hinder histamine release by skillfully obstructing the activation of mast cells and other cellular entities. Notably, they navigate this task without...
G-Protein Gated Ion Channels01:21

G-Protein Gated Ion Channels

GPCRs are primarily responsible for our sense of smell, taste, and vision.  The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
Sensory organs,...
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
Cholinergic Receptors: Muscarinic01:25

Cholinergic Receptors: Muscarinic

The pharmacological actions of acetylcholine are elicited via its binding to two families of cholinergic receptors or cholinoceptors, namely, muscarinic and nicotinic receptors. Muscarinic receptors are G protein-coupled receptors and have five subtypes, M1–M5. All mAChR subtypes are activated by acetylcholine and blocked by the antagonist, atropine. 
The subtypes M1, M3, and M5 couple with the Gq subunit and activate the phospholipase C (PLC) activity, mobilizing intracellular Ca2+. Activation...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The role of TRP channels in lung fibrosis: Mechanisms and therapeutic potential.

The international journal of biochemistry & cell biology·2024
Same author

Dynamic hyperinflation in patients with severe asthma compared to healthy adults.

Pulmonology·2023
Same author

Evaluation of Pirfenidone and Nintedanib in a Human Lung Model of Fibrogenesis.

Frontiers in pharmacology·2021
Same author

Validation of antibodies for the specific detection of human TRPA1.

Scientific reports·2019
Same author

Increased β2-adrenoceptor phosphorylation in airway smooth muscle in severe asthma: possible role of mast cell-derived growth factors.

Clinical and experimental immunology·2018
Same author

A randomised pragmatic trial of corticosteroid optimization in severe asthma using a composite biomarker algorithm to adjust corticosteroid dose versus standard care: study protocol for a randomised trial.

Trials·2018

Related Experiment Video

Updated: May 12, 2026

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis
16:01

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis

Published on: January 26, 2015

Ion channels regulating mast cell biology.

I Ashmole1, P Bradding

  • 1Department of Infection, Immunity and Inflammation, Institute for Lung Heath, Glenfield Hospital, University of Leicester, Leicester, LE3 9QP, UK.

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
|April 23, 2013
PubMed
Summary

Mast cells are key to asthma and allergies. Ion channels control calcium influx, essential for releasing mediators that cause allergic reactions.

More Related Videos

Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice
09:07

Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice

Published on: May 27, 2015

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
11:31

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

Published on: July 4, 2018

Related Experiment Videos

Last Updated: May 12, 2026

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis
16:01

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis

Published on: January 26, 2015

Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice
09:07

Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice

Published on: May 27, 2015

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
11:31

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

Published on: July 4, 2018

Area of Science:

  • Immunology
  • Cell Biology
  • Physiology

Background:

  • Mast cells are central to asthma and allergic conditions.
  • Mast cell activation releases mediators like histamine, leukotrienes, and cytokines.
  • Extracellular calcium influx is critical for mast cell mediator release.

Purpose of the Study:

  • To review ion channel expression and function in mast cells.
  • To emphasize the interaction of ion channels in regulating calcium signaling.
  • To provide an overview of current knowledge on ion channels in mast cell biology.

Main Methods:

  • Literature review of ion channel expression and function in mast cells.
  • Analysis of the role of various ion channels (Ca2+, K+, Cl-, TRP) in mast cell activation.
  • Examination of the interplay between ion channels in regulating calcium signaling.

Main Results:

  • Ion channels, including Ca2+, K+, Cl-, and transient receptor potential (TRP) channels, are critical for mast cell function.
  • These channels regulate membrane potential and control calcium influx, which is essential for mediator release.
  • The interaction between different ion channels modulates calcium signaling pathways.

Conclusions:

  • Ion channels are crucial regulators of mast cell activation and mediator release.
  • Understanding ion channel interplay is key to comprehending mast cell pathophysiology in allergic diseases.
  • Targeting ion channels may offer therapeutic strategies for asthma and related conditions.