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Related Concept Videos

Chromatin Packaging01:32

Chromatin Packaging

Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...
Chromatin Packaging02:21

Chromatin Packaging

Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter? 
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order structures.
Generation of Straight or Branched Actin Filaments01:14

Generation of Straight or Branched Actin Filaments

The straight or branched structure formation of actin filaments is controlled by nucleating proteins such as the formins and Arp2/3 complex. Formin-mediated assembly results in straight filaments, whereas Arp2/3 protein complex-mediated assembly results in branched actin filaments.
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
The DNA Helix01:16

The DNA Helix

Overview
The DNA Helix01:07

The DNA Helix

Deoxyribonucleic acid, or DNA, is the genetic material responsible for passing traits from generation to generation in all organisms and most viruses. DNA is composed of two strands of nucleotides that wind around each other to form a spring-like structure called a double helix. However, the double helix is not perfectly symmetrical. Instead, there are regularly occurring grooves in the structure. The major groove occurs where the sugar-phosphate backbones are relatively far apart. This space...

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Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
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Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

Backbone-branched DNA building blocks for facile angular control in nanostructures.

Eduardo Paredes1, Xiaojuan Zhang, Harshad Ghodke

  • 1Department of Chemistry and Center for Nucleic Acids Science and Technology, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, United States.

ACS Nano
|April 23, 2013
PubMed
Summary

Researchers developed backbone-branched DNAs (bbDNAs) for precise control over DNA nanostructure junction angles. This breakthrough enables the creation of highly tunable, strain-free DNA architectures with predictable shapes and sizes.

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Last Updated: May 12, 2026

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
09:32

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Published on: April 12, 2019

Design and Synthesis of a Reconfigurable DNA Accordion Rack
07:44

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Published on: August 15, 2018

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

Area of Science:

  • DNA nanotechnology
  • Biomolecular engineering
  • Materials science

Background:

  • DNA nanotechnology utilizes nucleobase pairing for creating complex 2D and 3D nanostructures.
  • Current methods for controlling junction angles in DNA assemblies are limited, often relying on unstructured crossovers or non-DNA components.
  • Achieving precise angular control is crucial for designing sophisticated DNA-based architectures.

Purpose of the Study:

  • To introduce a general strategy for direct and tunable control over junction angles in DNA nanostructures.
  • To demonstrate the utility of backbone-branched DNAs (bbDNAs) as a novel building block for DNA nanoconstruction.
  • To expand the toolkit for creating strain-free, size- and shape-tunable DNA architectures.

Main Methods:

  • Development of backbone-branched DNAs (bbDNAs) as building blocks.
  • Utilizing bbDNAs to define specific angular vertices within DNA nanostructures.
  • Characterization of the resulting nanostructures to confirm angular control and structural integrity.

Main Results:

  • Successfully demonstrated a general strategy for precise control of junction angles in DNA nanostructures.
  • Showcased bbDNAs as versatile building blocks for constructing well-defined nanostructures.
  • Achieved strain-free DNA architectures with tunable size and shape through controlled angular vertices.

Conclusions:

  • Backbone-branched DNAs (bbDNAs) offer a novel and effective method for precise angular control in DNA nanostructures.
  • This approach provides a versatile platform for designing complex, customizable DNA-based nanomaterials.
  • The developed strategy significantly advances the field of DNA nanotechnology, enabling greater precision in nanostructure fabrication.