Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Raman Spectroscopy: Overview

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...

Raman Spectroscopy Instrumentation: Overview

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.

IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration

IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration

A covalently bonded heteronuclear diatomic molecule can be modeled as two vibrating masses connected by a spring. The vibrational frequency of the bond can be expressed using an equation derived from Hooke's law, which describes how the force applied to stretch or compress a spring is proportional to the displacement of the spring. In this case, the atoms behave like masses, and the bond acts like a spring.
According to Hooke's law, the vibrational frequency is directly proportional to the...

2D NMR: Overview of Homonuclear Correlation Techniques

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...

Molecular Shape and Polarity

Molecular Shape and Polarity

Dipole Moment of a Molecule

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Overcoming Artifacts: Polarized Raman of Individual Electrospun Fibers Under Immersion.

Applied spectroscopy·2026

Same author

Partial Least Squares Models for the Orientation Analysis of Electrospun Fibers by Raman Spectroscopy.

Applied spectroscopy·2026

Same author

Thermoresponsive Behaviors of Poly(<i>N</i>-methacryloyl glycinamide) and Poly(<i>N</i>-acryloyl glycinamide): Effect of Methacrylation.

The journal of physical chemistry. B·2025

Same author

Azatriangulene-Based Conductive C═C Linked Covalent Organic Frameworks with Near-Infrared Emission.

Advanced materials (Deerfield Beach, Fla.)·2024

Same author

Engineered Robust Hydrophobic/Hydrophilic Nanofibrous Scaffolds with Drug-Eluting, Antioxidant, and Antimicrobial Capacity.

ACS applied bio materials·2024

Same author

Chlorophyllin-Containing Copolymers and Their Responsive Properties.

ACS applied bio materials·2023

Search research articles

Related Experiment Video

Updated: May 12, 2026

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy

Published on: December 1, 2023

Novel method for quantifying molecular orientation by polarized Raman spectroscopy: a comparative simulations study.

Marie Richard-Lacroix¹, Christian Pellerin

¹Department of Chemistry, Centre for Self-Assembled Chemical Structures, University of Montreal, Montreal, Quebec, Canada H3C 3J7.

Applied Spectroscopy

|April 23, 2013

Summary

A new method, the most probable distribution (MPD) method, offers accurate molecular orientation quantification using polarized Raman spectroscopy. It overcomes limitations of the depolarization constant (DC) method by not requiring an isotropic sample or assuming a constant depolarization ratio.

More Related Videos

Resonance Raman Spectroscopy of Extreme Nanowires and Other 1D Systems

Resonance Raman Spectroscopy of Extreme Nanowires and Other 1D Systems

Published on: April 28, 2016

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

Related Experiment Videos

Last Updated: May 12, 2026

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy

Published on: December 1, 2023

Resonance Raman Spectroscopy of Extreme Nanowires and Other 1D Systems

Resonance Raman Spectroscopy of Extreme Nanowires and Other 1D Systems

Published on: April 28, 2016

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

Area of Science:

Materials Science
Spectroscopy
Physical Chemistry

Background:

Polarized Raman spectroscopy quantifies molecular orientation.
The depolarization constant (DC) method relies on a constant depolarization ratio.
DC method requires an isotropic sample for accurate ratio determination, limiting its applicability.

Purpose of the Study:

Introduce a novel method for molecular orientation quantification.
Overcome the limitations of the conventional DC method.
Improve the accuracy and applicability of orientation analysis in polarized Raman spectroscopy.

Main Methods:

Developed the most probable distribution (MPD) method.
MPD method is based on the hypothesis of the most probable population distribution.
MPD method does not require prior knowledge of the depolarization ratio.

Main Results:

Simulations demonstrate the wide applicability of the MPD method.
MPD method is effective across large sections of the 〈P2〉〈P4〉 diagram.
MPD method shows significant accuracy improvements over the DC method, especially when depolarization ratios vary.

Conclusions:

The MPD method provides a robust alternative for molecular orientation quantification.
MPD method eliminates the need for isotropic samples and assumptions about depolarization ratio constancy.
This advancement enhances the reliability of polarized Raman spectroscopy for material analysis.