Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Receptor Tyrosine Kinases

Receptor Tyrosine Kinases

Receptor tyrosine kinases or RTKs are membrane-bound receptors that phosphorylate specific tyrosine on protein substrates. RTKs regulate cellular growth, differentiation, survival, and migration. They contain an extracellular ligand binding domain, a transmembrane domain, and a cytosolic tail with intrinsic kinase activity. Several extracellular signaling molecules activate RTKs in one or more ways and relay the signal downstream. Ligands such as platelet-derived growth factor (PDGF) or...

Phosphorylation

Phosphorylation

The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Fluorothiazynes as SuFEx Ambiphiles: Interconversion to Amino-sulfurdiimidoyl Fluorides.

Angewandte Chemie (International ed. in English)·2025

Same author

Mixed Alkyl Aryl Phosphonates as Quenched Activity-Based Probes for Real-Time Imaging of Active Neutrophil Serine Proteases.

ACS chemical biology·2025

Same author

BDH2-driven lysosome-to-mitochondria iron transfer shapes ferroptosis vulnerability of the melanoma cell states.

Nature metabolism·2025

Same author

Sulfoxide-diazirine (SODA) as a Cleavable Photoaffinity Group To Enable the Identification of Photolabeled Modification Sites via LC-MS<sup>3</sup>.

Analytical chemistry·2025

Same author

Icosapent ethyl reduces arterial thrombosis by inhibition of cyclooxygenase-1-induced platelet reactivity.

Science translational medicine·2025

Same author

Polyamine depletion limits progression of acute leukaemia.

International journal of cancer·2025

Search research articles

Related Experiment Video

Updated: May 12, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Probing functional tyrosines.

Steven H L Verhelst¹

¹Lehrstuhl für Chemie der Biopolymere, Technische Universität München, Weihenstephaner Berg 3, 85354 Freising, Germany. verhelst@wzw.tum.de

Chemistry & Biology

|April 23, 2013

Summary

This summary is machine-generated.

Reactivity probes identified functional tyrosine residues in glutathione S-transferases. Sulfonyl fluoride derivatives were used to tag and find these hyperreactive amino acid sites.

More Related Videos

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

Published on: August 10, 2012

Nitropeptide Profiling and Identification Illustrated by Angiotensin II

Nitropeptide Profiling and Identification Illustrated by Angiotensin II

Published on: June 16, 2019

Related Experiment Videos

Last Updated: May 12, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

Published on: August 10, 2012

Nitropeptide Profiling and Identification Illustrated by Angiotensin II

Nitropeptide Profiling and Identification Illustrated by Angiotensin II

Published on: June 16, 2019

Area of Science:

Biochemistry
Chemical Biology

Background:

Reactivity probes are essential tools for identifying hyperreactive amino acid residues within proteomes.
These probes consist of a reactive electrophile and a purification handle.

Discussion:

Gu et al. employed sulfonyl fluoride derivatives as reactivity probes.
The study focused on identifying functional tyrosine residues in various glutathione S-transferase classes.

Key Insights:

Sulfonyl fluoride probes effectively target and identify specific functional tyrosine residues.
This method allows for the characterization of enzyme active sites and mechanisms.

Outlook:

This approach can be extended to study other enzyme families and their functional residues.
Further development of reactivity probes will enhance proteome-wide functional site identification.