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Related Concept Videos

Regulated Protein Degradation02:58

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Regulated Protein Degradation02:58

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
The Proteasome01:13

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3 (ubiquitin...
The Proteasome02:18

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA molecules by RNA...

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Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae
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Published on: February 16, 2015

Photoswitchable protein degradation: a generalizable control module for cellular function?

Evan Mills1, Kevin Truong

  • 1Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, Ontario, M5S 3G9, Canada. e.mills@mail.utoronto.ca

Chemistry & Biology
|April 23, 2013
PubMed
Summary
This summary is machine-generated.

Researchers developed a photosensitive degron (psd) for light-controlled protein degradation. This technology precisely regulates yeast cell cycles using light, offering new possibilities for biological research.

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Area of Science:

  • Biochemistry and Molecular Biology
  • Cell Biology
  • Synthetic Biology

Background:

  • Protein degradation is a fundamental cellular process.
  • Controlling protein stability is crucial for understanding cellular functions.
  • Existing methods for protein degradation often lack spatiotemporal control.

Discussion:

  • Renicke et al. engineered a novel photosensitive degron (psd) by fusing the LOV2 domain with a protein degradation sequence.
  • The psd enables light-inducible protein degradation in yeast.
  • This system allows for precise control over protein stability using external light stimuli.

Key Insights:

  • The psd system demonstrates effective light-dependent protein degradation in a living organism.
  • Fusion of psd to cell-cycle-dependent proteins allowed for light-mediated control of the cell cycle.
  • The study highlights the potential of optogenetic tools for precise biological regulation.

Outlook:

  • The psd technology could be applied to other model organisms and cellular processes.
  • Further development may lead to advanced optogenetic tools for research and therapeutics.
  • This work opens avenues for spatiotemporal control of protein function in complex biological systems.