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Receptor-mediated Endocytosis01:38

Receptor-mediated Endocytosis

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Related Experiment Video

Updated: May 12, 2026

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy
06:15

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy

Published on: August 18, 2018

Fluorogenic probe for constitutive cellular endocytosis.

Michael N Levine1, Trish T Hoang, Ronald T Raines

  • 1Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.

Chemistry & Biology
|April 23, 2013
PubMed
Summary
This summary is machine-generated.

We developed a novel molecular probe to measure endocytosis, a key cellular process. This probe revealed that human breast cancer cells exhibit significantly higher rates of endocytosis compared to normal cells, suggesting a potential therapeutic target.

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Last Updated: May 12, 2026

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy
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Quantification of Efferocytosis by Single-cell Fluorescence Microscopy

Published on: August 18, 2018

Imaging FITC-dextran as a Reporter for Regulated Exocytosis
04:50

Imaging FITC-dextran as a Reporter for Regulated Exocytosis

Published on: June 20, 2018

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cancer Research

Background:

  • Endocytosis is a vital cellular mechanism for nutrient uptake and signaling in eukaryotic cells.
  • Quantifying endocytosis rates is crucial for understanding cellular functions and disease states.
  • Existing methods for measuring endocytosis may have limitations in sensitivity or specificity.

Purpose of the Study:

  • To develop and validate a novel molecular probe for quantifying cellular endocytosis.
  • To investigate differences in endocytosis rates between human breast cancer cells and noncancerous cells.
  • To explore the potential of targeting endocytosis for cancer therapy.

Main Methods:

  • Development of a lipid-conjugated molecular probe with an enzyme-activatable "trimethyl lock" moiety.
  • The probe is designed to be non-fluorescent until activated within endosomes by cellular esterases.
  • Application of the probe in human breast cancer cells and matched noncancerous cells to measure endocytosis rates.

Main Results:

  • The developed molecular probe successfully quantifies endocytosis by becoming fluorescent upon cellular uptake and activation.
  • Human breast cancer cells demonstrated significantly higher constitutive endocytosis rates compared to matched noncancerous cells.
  • The findings suggest a distinct endocytic phenotype in cancer cells.

Conclusions:

  • The novel trimethyl lock-activated probe provides a sensitive tool for measuring endocytosis.
  • Accelerated endocytosis is a potential distinguishing characteristic of human breast cancer cells.
  • Targeting the enhanced endocytosis in cancer cells represents a promising avenue for chemotherapeutic intervention.