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Related Concept Videos

Regulated Protein Degradation02:58

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Regulated Protein Degradation02:58

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA molecules by RNA...
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA molecules by RNA...
Directing Proteins to the Rough Endoplasmic Reticulum01:34

Directing Proteins to the Rough Endoplasmic Reticulum

The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.

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Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation
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Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Published on: October 4, 2024

A LOV2 domain-based optogenetic tool to control protein degradation and cellular function.

Christian Renicke1, Daniel Schuster, Svetlana Usherenko

  • 1Department of Biology/Genetics, Philipps-Universität Marburg, Karl-von-Frisch-Strasse 8, 35043 Marburg, Germany.

Chemistry & Biology
|April 23, 2013
PubMed
Summary

Researchers engineered a synthetic photosensitive degron (psd) module to control protein stability using light. This breakthrough enables light-switchable biological processes in non-plant organisms for diverse applications.

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Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

Area of Science:

  • Biotechnology
  • Synthetic Biology
  • Molecular Biology

Background:

  • Light perception is crucial for plant responses, involving proteolysis of transcriptional regulators.
  • Controlling protein stability is key for understanding and manipulating biological processes.

Purpose of the Study:

  • To develop a generic photosensitive degron (psd) module for synthetic light control of protein stability.
  • To demonstrate the functionality and versatility of the psd module in non-plant organisms.

Main Methods:

  • Combined the light-reactive LOV2 domain of Arabidopsis thaliana phot1 with the murine cODC1 degradation sequence.
  • Demonstrated functionality in the model organism Saccharomyces cerevisiae.
  • Utilized in silico modeling to understand psd module behavior.

Main Results:

  • Successfully engineered a versatile psd module for light-regulated protein degradation.
  • Showcased applications including conditional mutants, cell growth patterning, and yeast photography.
  • Validated the psd module's effectiveness in a non-plant system.

Conclusions:

  • The psd module effectively transfers light-regulated degradation principles to non-plant organisms.
  • This technology offers significant potential for biotechnological and biomedical applications.
  • Enables light-switchable control over a wide range of biological processes.