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LIBS analysis of artificial calcified tissues matrices
M A Kasem1, J J Gonzalez, R E Russo
1National Institute of Laser Enhanced Science (NILES), Cairo University, Giza, Egypt.
Talanta
|April 23, 2013
Summary
UV laser-induced breakdown spectroscopy (LIBS) offers improved reproducibility and reduced matrix effects for analyzing calcified tissues. Bone ash is a suitable reference material for calibration using UV-LIBS.
Area of Science:
- Analytical Chemistry
- Materials Science
- Biomedical Engineering
Background:
- Reproducibility in laser-based analytical methods, like Laser-Induced Breakdown Spectroscopy (LIBS), is crucial for accurate elemental quantification.
- Calcified tissues, such as bone, present unique challenges due to their texture, potentially impacting ablation stability and measurement reproducibility.
- Stable plasma conditions and consistent experimental parameters are essential for reliable LIBS analysis.
Purpose of the Study:
- To investigate the effect of excitation wavelength (UV vs. IR) on LIBS analysis of different calcium-based matrices.
- To compare the performance of UV (266 nm) and IR (1064 nm) LIBS for analyzing artificial reference samples mimicking calcified tissues.
- To evaluate the suitability of bone ash as a reference material for LIBS calibration of calcified tissues.
Main Methods:
- Fabrication of three artificial reference sample sets: CaCO3, bone ash, and Ca hydroxyapatite.
- Comparative analysis using UV (266 nm) and IR (1064 nm) LIBS under identical experimental conditions.
- Evaluation of plasma parameters, reproducibility, precision, linear fitting, and matrix effects.
Main Results:
- UV-LIBS demonstrated superior reproducibility, precision, and stability of plasma conditions compared to IR-LIBS.
- UV-LIBS exhibited better linear fitting and a reduction in matrix effects across the tested sample types.
- Bone ash emerged as a promising reference material for calcified tissue analysis using UV-LIBS.
Conclusions:
- UV (266 nm) excitation is more advantageous for LIBS analysis of calcium-based matrices, including calcified tissues, owing to enhanced analytical performance.
- The use of bone ash as a reference material with UV-LIBS offers a viable solution for accurate elemental calibration in calcified tissues.
- Optimizing LIBS parameters, particularly the excitation wavelength, is critical for overcoming challenges in analyzing complex biological samples.
