Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Bone Matrix01:18

The Bone Matrix

Bone contains a relatively small number of cells entrenched in a matrix of collagen fibers that provide an adherent surface for inorganic salt crystals. Both components of the matrix, organic and inorganic, contribute to the unusual properties of bone. Without collagen, bones would be brittle and shatter easily. Without mineral crystals, bones would flex and provide little support. This can be observed by an experiment: when the minerals of a bone are dissolved by soaking the bone in acid or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Photobiomodulation of avian embryos by red laser.

Lasers in medical science·2020
Same author

Study of the feeding effect on recent and ancient bovine bones by nanoparticle-enhanced laser-induced breakdown spectroscopy and chemometrics.

Journal of advanced research·2019
Same author

WITHDRAWN: Effect of He-Ne laser irradiation on embryonic development in chicken eggs.

Progress in biophysics and molecular biology·2019
Same author

Evaluation of proteins in sheep colostrum via laser-induced breakdown spectroscopy and multivariate analysis.

Journal of advanced research·2018
Same author

Detachment of Cu (II) and Co (II) ions from synthetic wastewater via adsorption on <i>Lates niloticus</i> fish bones using LIBS and XRF.

Journal of advanced research·2018
Same author

WITHDRAWN: An extremely low frequency-weak magnetic field can induce alterations in a biological system: A case study in chick embryo development.

Progress in biophysics and molecular biology·2018

Related Experiment Video

Updated: May 12, 2026

Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation
08:43

Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation

Published on: May 31, 2016

LIBS analysis of artificial calcified tissues matrices.

M A Kasem1, J J Gonzalez, R E Russo

  • 1National Institute of Laser Enhanced Science (NILES), Cairo University, Giza, Egypt.

Talanta
|April 23, 2013
PubMed
Summary

UV laser-induced breakdown spectroscopy (LIBS) offers improved reproducibility and reduced matrix effects for analyzing calcified tissues. Bone ash is a suitable reference material for calibration using UV-LIBS.

More Related Videos

Comprehensive Characterization of Tissue Mineralization in an Ex Vivo Model
07:29

Comprehensive Characterization of Tissue Mineralization in an Ex Vivo Model

Published on: September 27, 2024

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix
09:40

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix

Published on: January 4, 2017

Related Experiment Videos

Last Updated: May 12, 2026

Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation
08:43

Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation

Published on: May 31, 2016

Comprehensive Characterization of Tissue Mineralization in an Ex Vivo Model
07:29

Comprehensive Characterization of Tissue Mineralization in an Ex Vivo Model

Published on: September 27, 2024

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix
09:40

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix

Published on: January 4, 2017

Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Biomedical Engineering

Background:

  • Reproducibility in laser-based analytical methods, like Laser-Induced Breakdown Spectroscopy (LIBS), is crucial for accurate elemental quantification.
  • Calcified tissues, such as bone, present unique challenges due to their texture, potentially impacting ablation stability and measurement reproducibility.
  • Stable plasma conditions and consistent experimental parameters are essential for reliable LIBS analysis.

Purpose of the Study:

  • To investigate the effect of excitation wavelength (UV vs. IR) on LIBS analysis of different calcium-based matrices.
  • To compare the performance of UV (266 nm) and IR (1064 nm) LIBS for analyzing artificial reference samples mimicking calcified tissues.
  • To evaluate the suitability of bone ash as a reference material for LIBS calibration of calcified tissues.

Main Methods:

  • Fabrication of three artificial reference sample sets: CaCO3, bone ash, and Ca hydroxyapatite.
  • Comparative analysis using UV (266 nm) and IR (1064 nm) LIBS under identical experimental conditions.
  • Evaluation of plasma parameters, reproducibility, precision, linear fitting, and matrix effects.

Main Results:

  • UV-LIBS demonstrated superior reproducibility, precision, and stability of plasma conditions compared to IR-LIBS.
  • UV-LIBS exhibited better linear fitting and a reduction in matrix effects across the tested sample types.
  • Bone ash emerged as a promising reference material for calcified tissue analysis using UV-LIBS.

Conclusions:

  • UV (266 nm) excitation is more advantageous for LIBS analysis of calcium-based matrices, including calcified tissues, owing to enhanced analytical performance.
  • The use of bone ash as a reference material with UV-LIBS offers a viable solution for accurate elemental calibration in calcified tissues.
  • Optimizing LIBS parameters, particularly the excitation wavelength, is critical for overcoming challenges in analyzing complex biological samples.