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Related Experiment Video

Updated: May 12, 2026

Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides
10:27

Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides

Published on: July 14, 2015

Functionalized diamond nanopowder for phosphopeptides enrichment from complex biological fluids.

Dilshad Hussain1, Muhammad Najam-ul-Haq, Fahmida Jabeen

  • 1Division of Analytical Chemistry, Institute of Chemical Sciences, Bahauddin Zakariya University, Multan 60800, Pakistan.

Analytica Chimica Acta
|April 23, 2013
PubMed
Summary

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Diamond nanopowder serves as a versatile material for both phosphopeptide enrichment and sample desalting. This functionalized diamond shows promise for identifying cancer biomarkers and profiling complex biological mixtures.

Area of Science:

  • Biomaterials Science
  • Analytical Chemistry
  • Proteomics

Background:

  • Diamond exhibits excellent biocompatibility and affinity for biomolecules, making it suitable for separation sciences.
  • Existing separation techniques can be enhanced by novel materials with multifunctional properties.

Purpose of the Study:

  • To functionalize diamond nanopowder for Immobilized Metal Ion Affinity Chromatography (IMAC) and Reversed-Phase (C-18) applications.
  • To evaluate diamond-based materials for phosphopeptide enrichment, sample desalting, and human serum profiling.
  • To explore the potential of diamond-IMAC in identifying cancer biomarkers.

Main Methods:

  • Diamond nanopowder was derivatized into IMAC and C-18 media.
  • Functionalization was characterized using Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), and energy dispersive X-ray (EDX) spectroscopy.

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Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer

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Chemical Affinity-Based Isolation of Extracellular Vesicles from Biofluids for Proteomics and Phosphoproteomics Analysis
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Chemical Affinity-Based Isolation of Extracellular Vesicles from Biofluids for Proteomics and Phosphoproteomics Analysis

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Last Updated: May 12, 2026

Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides
10:27

Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides

Published on: July 14, 2015

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer
12:23

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer

Published on: August 2, 2018

Chemical Affinity-Based Isolation of Extracellular Vesicles from Biofluids for Proteomics and Phosphoproteomics Analysis
09:28

Chemical Affinity-Based Isolation of Extracellular Vesicles from Biofluids for Proteomics and Phosphoproteomics Analysis

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  • Diamond-IMAC was applied to phosphopeptide enrichment from various biological samples, including spiked human serum and gall bladder carcinoma serum.

    • Main Results:

    • The synthesized IMAC-diamond immobilized with Fe(3+) and La(3+) ions demonstrated selectivity for phosphopeptide enrichment.
    • Diamond nanopowder effectively served as a Reversed-Phase (C-18) medium for desalting complex mixtures.
    • The study successfully applied diamond-IMAC to gall bladder carcinoma serum samples for potential biomarker discovery.

    Conclusions:

    • Functionalized diamond nanopowder is a multifunctional separation medium with applications in proteomics and life science research.
    • Diamond-based materials offer a promising platform for phosphopeptide enrichment and biomarker identification in complex biological samples.
    • This approach holds potential for cancer protein profiling, aiding in diagnosis and biomarker discovery.