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Related Concept Videos

mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...

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Related Experiment Video

Updated: May 12, 2026

Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts
07:03

Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts

Published on: January 2, 2018

Signaling pathways that control mRNA turnover.

Roopa Thapar1, Andria P Denmon

  • 1Hauptman-Woodward Medical Research Institute, 700 Ellicott Street, Buffalo, NY 14203, USA. rthapar@hwi.buffalo.edu

Cellular Signalling
|April 23, 2013
PubMed
Summary
This summary is machine-generated.

Cells rapidly respond to stimuli by regulating mRNA decay through signaling pathways. Posttranslational modifications like phosphorylation and ubiquitination are key to controlling gene expression via mRNA turnover.

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Related Experiment Videos

Last Updated: May 12, 2026

Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts
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Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts

Published on: January 2, 2018

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
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Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
09:44

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

Published on: March 3, 2015

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Gene Regulation

Background:

  • Cellular genomes are primarily regulated by transcription and mRNA decay.
  • mRNA turnover, influenced by signaling networks, enables rapid cellular responses to external stimuli.
  • Posttranslational modifications (PTMs) of RNA-binding proteins are crucial for dynamic ribonucleoprotein complex remodeling and mRNA decay.

Purpose of the Study:

  • To review recent findings on how signaling pathways and cell cycle checkpoints impact mRNA turnover.
  • To elucidate the role of PTMs in regulating gene expression through mRNA decay.

Main Methods:

  • Literature review of recent research findings.
  • Analysis of signaling pathways and cell cycle checkpoints.
  • Focus on phosphorylation, ubiquitination, and arginine methylation in mRNA turnover.

Main Results:

  • Signaling pathways utilize PTMs (phosphorylation, ubiquitination, etc.) to modulate RNA-binding proteins.
  • These modifications facilitate the rapid remodeling of ribonucleoprotein complexes.
  • PTMs are essential for triggering mRNA decay and thus regulating gene expression.

Conclusions:

  • Posttranslational modifications are critical mediators between signaling pathways and mRNA turnover.
  • Understanding these modifications provides fundamental insights into gene expression control.
  • The review highlights the interplay between cell signaling, cell cycle, and mRNA decay mechanisms.