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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
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lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Translational Regulation01:29

Translational Regulation

Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...

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Updated: May 12, 2026

Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
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Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting

Published on: March 17, 2023

RNA-seq identified a super-long intergenic transcript functioning in adipogenesis.

Fan Yi1, Feng Yang, Xiaoqiao Liu

  • 1Laboratory of Nucleic Acid Technology and Laboratory of State Key Laboratory of Natural and Biomimetic Drugs, Institute of Molecular Medicine, School of Pharmaceutical Sciences, Peking University, Beijing, China.

RNA Biology
|April 23, 2013
PubMed
Summary
This summary is machine-generated.

Researchers explored polyadenylation-minus (polyA-minus) RNAs during fat cell differentiation. They identified novel transcripts, including a long non-coding RNA, that are upregulated and fat tissue-specific, suggesting a role in adipogenesis.

Keywords:
adipogenesishigh-throughput sequencinglarge intergenic non-coding RNAobesitypolyA-minus RNA

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Isolation of Nuclei from Human Intermuscular Adipose Tissue and Downstream Single-Nuclei RNA Sequencing
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Isolation of Nuclei from Human Intermuscular Adipose Tissue and Downstream Single-Nuclei RNA Sequencing

Published on: May 3, 2024

Area of Science:

  • Molecular Biology
  • Genomics
  • Cell Biology

Background:

  • RNA transcripts are classified into polyA-plus and polyA-minus based on the presence or absence of a polyA tail.
  • While polyA-minus RNAs are increasingly recognized for their physiological and pathological importance, their role in adipogenesis remains understudied.
  • Adipogenesis, the process of fat cell differentiation, involves complex gene expression regulation.

Purpose of the Study:

  • To systematically analyze the expression and potential function of polyA-minus RNAs during adipogenesis.
  • To identify novel transcripts and regulatory elements involved in fat cell differentiation.
  • To characterize the expression patterns of these transcripts in adipose tissue.

Main Methods:

  • Dynamic expressional profiling of 3T3-L1 cells during induced differentiation.
  • Identification and characterization of novel intergenic transcripts.
  • Analysis of differentiation-synchronized expression and fat tissue-specific expression patterns.

Main Results:

  • Thousands of novel intergenic transcripts were identified.
  • Differentiation-synchronized expression was characterized for many transcripts.
  • Several large intergenic transcripts showed significant upregulation (>19-fold) during differentiation and exhibited fat tissue-specific expression, including an adipogenesis-associated long non-coding RNA.

Conclusions:

  • PolyA-minus RNAs, particularly long intergenic transcripts, play a role in adipogenesis.
  • A novel super-long intergenic transcript has been characterized for its function in fat cell differentiation.
  • These findings contribute to understanding the regulatory landscape of adipogenesis beyond polyadenylated RNAs.