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Thrombin aptasensing with inherently electroactive graphene oxide nanoplatelets as labels
Adeline Huiling Loo1, Alessandra Bonanni, Martin Pumera
1Division of Chemistry & Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore, 637371, Singapore.
Nanoscale
|April 23, 2013
Summary
Graphene oxide nanoplatelets serve as electroactive labels for detecting thrombin. This novel aptasensing method offers high sensitivity and selectivity for biosensing applications.
Area of Science:
- Materials Science
- Analytical Chemistry
- Biotechnology
Background:
- Graphene materials are widely used in biosensing platforms.
- A novel application of graphene oxide nanoplatelets as electroactive labels is proposed.
Purpose of the Study:
- To utilize graphene oxide nanoplatelets as inherently electroactive labels for aptasensing of thrombin.
- To establish a sensitive and selective electrochemical detection method for thrombin.
Main Methods:
- Graphene oxide nanoplatelets were employed as electroactive labels.
- Electrochemical reduction of graphene oxide nanoplatelets provided a detection signal.
- Aptasensing assay was developed for thrombin detection.
Main Results:
- Thrombin was detected in the concentration range of 3 pM–0.3 μM.
- The method demonstrated good selectivity against bovine serum albumin, immunoglobulin G, and avidin.
- Each graphene oxide nanoplatelet provided a significant reduction signal due to electron acceptance.
Conclusions:
- Graphene oxide nanoplatelets function effectively as electroactive labels for aptasensing.
- This approach offers a viable alternative to metallic nanoparticles in electrochemical biosensing.
- The developed method shows promise for sensitive and selective thrombin detection.
