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Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
Published on: February 7, 2018
Morphologic assessment of oxidative damage: A review
1Pathology and Laboratory Medicine Service, Veterans Administration Hospital, Madison, WI ; Department of Pathology and Laboratory Medicine, University of Wisconsin Medical School, Madison, WI ; Room A-35, VA Hospital, 2500 Overlook Terrace, Madison, WI.
Journal of the American Aging Association
|April 23, 2013
Summary
Researchers developed new microscopy techniques to pinpoint oxidative damage in cells. This helps understand diseases like aging, neurodegeneration, and cancer by showing where damage occurs.
Area of Science:
- Biochemistry
- Cell Biology
- Pathology
Background:
- Oxidative damage and altered antioxidant enzyme activity are linked to aging and diseases like cancer and neurodegeneration.
- Understanding the cellular and subcellular sites of oxidative damage is crucial for disease research.
Purpose of the Study:
- To develop and apply quantitative microscopy techniques for localizing oxidative damage products within cells.
- To investigate the cellular and subcellular distribution of oxidative damage in various pathological conditions.
Main Methods:
- Development of quantitative light and electron microscopy immunogold techniques.
- Utilizing specific antibodies targeting oxidative damage products.
- Application of these methods to study different pathological processes.
Main Results:
- Successful localization and quantitation of oxidative damage products at cellular and subcellular levels.
- Demonstration of the technique's applicability across diverse pathological conditions.
- Insights into the specific sites of oxidative damage in disease.
Conclusions:
- Quantitative microscopy immunogold techniques are effective for mapping oxidative damage.
- These methods provide valuable insights into the pathogenesis of aging-related diseases.
- The findings advance our understanding of oxidative stress in disease.
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