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Non-destructive SPE-UPLC-based Quantification of Aflatoxins and Stilbenoid Phytoalexins in Single Peanut (Arachis spp.) Seeds
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Non-destructive SPE-UPLC-based Quantification of Aflatoxins and Stilbenoid Phytoalexins in Single Peanut (Arachis spp.) Seeds

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A rapid extraction method for detecting aflatoxin producing isolates.

M A Moreno1, M Del Carmen Ramos, G Suàrez

  • 1Unidad Docente de Microbiologia, Departamento de Patologia Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense de Madrid, E-28040, Madrid, Spain.

Mycotoxin Research
|April 23, 2013
PubMed
Summary
This summary is machine-generated.

A new, rapid method screens aflatoxin-producing potential in Aspergillus flavus using moist wheat and chloroform extraction. This technique efficiently analyzes fungal isolates from animal feed for mycotoxin risk assessment.

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RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem

Published on: December 21, 2015

Area of Science:

  • Mycology
  • Food Safety
  • Analytical Chemistry

Background:

  • Aflatoxins are toxic secondary metabolites produced by Aspergillus species.
  • Aspergillus flavus group fungi are significant producers of aflatoxins, posing risks to animal feed and human health.
  • Accurate and rapid screening methods are crucial for managing aflatoxin contamination.

Purpose of the Study:

  • To develop and validate a rapid extraction method for assessing the aflatoxin-producing potential of Aspergillus flavus group isolates.
  • To provide a tool for efficient screening of fungal contamination in animal feed.

Main Methods:

  • Utilized a moist wheat medium for fungal growth.
  • Employed a rapid extraction process involving approximately five infected grains with 2 mL of chloroform.
  • Analyzed extracts using thin-layer chromatography (TLC).

Main Results:

  • The described method demonstrated effectiveness in screening aflatoxin-producing potential.
  • Successfully applied to 95 Aspergillus flavus isolates obtained from animal feed samples.
  • Indicated the method's suitability for routine laboratory application.

Conclusions:

  • The developed rapid extraction and TLC method is a viable tool for screening aflatoxin-producing Aspergillus flavus.
  • This method offers a practical approach for mycotoxin risk assessment in animal feed.
  • Facilitates quicker identification of potentially hazardous fungal isolates.