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Related Experiment Video

Updated: May 12, 2026

Engineering Cell-permeable Protein
21:08

Engineering Cell-permeable Protein

Published on: December 28, 2009

Bioreducible polypeptide containing cell-penetrating sequence for efficient gene delivery.

Si Chen1, Kai Han, Juan Yang

  • 1Key Laboratory of Biomedical Polymers (The Ministry of Education) Department of Chemistry, Wuhan University, Wuhan 430072, China.

Pharmaceutical Research
|April 23, 2013
PubMed
Summary

New bioreducible polypeptides show promise as non-viral gene vectors. These gene delivery systems exhibit excellent DNA binding and cellular uptake, with xPolyK6-R82 demonstrating the highest transfection efficiency.

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Area of Science:

  • Biomaterials Science
  • Gene Therapy
  • Nanotechnology

Background:

  • Non-viral gene vectors are crucial for safe and effective gene therapy.
  • Developing efficient and low-cytotoxicity gene delivery systems remains a challenge.

Purpose of the Study:

  • To design and evaluate novel polypeptide-based gene vectors.
  • To determine the gene delivery efficiency and cellular uptake of these vectors.

Main Methods:

  • Polypeptides were synthesized via oxidative polymerization of DNA-condensing and cell-penetrating peptides.
  • Cytotoxicity was assessed using MTT assay.
  • Complexation, particle size, zeta potential, morphology, in vitro transfection efficiency, and cellular uptake were characterized.

Main Results:

  • All synthesized vectors effectively condensed plasmid DNA (pDNA) with low cytotoxicity.
  • Transfection efficiency correlated with vector composition, particularly the content of cell-penetrating peptide (CPP).
  • xPolyK6-R82 exhibited the highest transfection efficiency and superior cellular uptake.

Conclusions:

  • Bioreducible polypeptides incorporating an optimal amount of CPP are effective non-viral gene vectors.
  • Oxidative polymerization enhances transfection efficacy.
  • These novel vectors hold significant potential for gene therapy applications.