Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 12, 2026

Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test
05:25

Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test

Published on: July 14, 2023

A multicentre study comparing two methods for serum free light chain analysis.

R J Lock1, R Saleem, E G Roberts

  • 1Immunology and Immunogenetics, Pathology Sciences, North Bristol NHS Trust, Southmead Hospital, Westbury-on-Trym, Bristol BS10 5NB, UK. Robert.lock@nbt.nhs.uk

Annals of Clinical Biochemistry
|April 23, 2013
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Description and predictors of self-determination in males and females with fragile X syndrome on the verge of adulthood.

Journal of intellectual disability research : JIDR·2024
Same author

The impact of obstructive sleep apnea on exercise capacity in a cardiac rehabilitation program.

Sleep & breathing = Schlaf & Atmung·2022
Same author

Analysis of antioxidants in water striders (Hemiptera: Gerridae) as bioindicator of water pollution.

Brazilian journal of biology = Revista brasleira de biologia·2022
Same author

An exact solution approach to warm inflation using Tsallis and Barrow holographic dark energy entropy within Rastall gravity.

European physical journal plus·2022
Same author

Experiences of Parents of Specialist Peer Mentored Autistic University Students.

Developmental neurorehabilitation·2021
Same author

Contralateral Effects of Unilateral Strength and Skill Training: Modified Delphi Consensus to Establish Key Aspects of Cross-Education.

Sports medicine (Auckland, N.Z.)·2020
Same journal

Routine laboratory triage for NT-proBNP elevation may reflect cardiorenal frailty as much as heart failure specificity.

Annals of clinical biochemistry·2026
Same journal

From interpretable NT-proBNP prediction to equitable laboratory stewardship.

Annals of clinical biochemistry·2026
Same journal

Serum Sclerostin Levels in Children with Osteogenesis Imperfecta.

Annals of clinical biochemistry·2026
Same journal

Population-Specific HOMA-IR Reference Ranges in Iraq: Defining Insulin Sensitivity in a Middle Eastern Cohort.

Annals of clinical biochemistry·2026
Same journal

Optimizing Laboratory Quality with Sigma Metrics: Application of CLIA 2024 Total Allowable Error Guidelines.

Annals of clinical biochemistry·2026
Same journal

Suspected thermal amplitude of cryoglobulin likely more clinically significant than size detected: A case report.

Annals of clinical biochemistry·2026
See all related articles

Two serum free light chain analysis kits show poor agreement in routine laboratory testing. These diagnostic assays cannot be used interchangeably, particularly for monitoring patient treatment responses in monoclonal gammopathies.

Area of Science:

  • Clinical Chemistry
  • Immunodiagnostics

Background:

  • Serum free light chain (sFLC) analysis is crucial for diagnosing monoclonal gammopathies.
  • Historically, a single kit dominated the UK market.
  • A new monoclonal antisera-based kit has been introduced, necessitating performance comparison.

Purpose of the Study:

  • To compare the clinical performance of two commercially available sFLC assay kits.
  • To evaluate the interchangeability of the Freelite and N Latex FLC assays in routine laboratory settings.

Main Methods:

  • A comparative study was conducted across four routine laboratories.
  • 327 patient samples, predominantly from B-cell lymphoproliferative disease patients (79%), were analyzed.
  • Both Freelite (Binding Site) and N Latex FLC (Siemens) kits were used according to manufacturer instructions.

More Related Videos

A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases
06:50

A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases

Published on: May 29, 2020

Related Experiment Videos

Last Updated: May 12, 2026

Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test
05:25

Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test

Published on: July 14, 2023

A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases
06:50

A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases

Published on: May 29, 2020

Main Results:

  • Poor qualitative agreement was observed between the two kits: 81% for kappa and 74% for lambda chains.
  • A significant discordance rate of 5% was found across the analyzed samples.
  • Data availability varied slightly, with 313 samples for kappa and 324 for lambda chains.

Conclusions:

  • The two sFLC assay kits exhibit substantial performance differences in clinical practice.
  • These assays are not interchangeable, posing challenges for consistent patient monitoring.
  • Clinical decisions, especially regarding therapy response, should consider the specific assay used.