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Published on: February 15, 2019
Genetic background of ochratoxin A production inPenicillium
A Karolewiez1, C Bogs, R Geisen
1Federal Research Centre for Nutrition and Food, Location Karisruhe, Haid-und-Neu-Str. 9, D-76131, Karisruhe.
Abstract:
A part of the gene cluster responsible for the production of ochratoxin A inP. nordicum has been characterised recently. The analysed DNA region contains three putative genes: an alcaline proteinase (aspPN), a non-ribosomal peptide synthetase (npsPN) and an polyketide synthase (otapksPN). The last two genes are putative genes of the ochratoxin A biosynthetic pathway. Interestingly theotapksPN gene is present in this form only inP. nordicum but not inP. verrucosum indicating genetic differences between both ochratoxin A producingPenicillium species. The genes in ochratoxin A producingAspergillus species seems to be completely different. It has been demonstrated that thenpsPN gene is actively transcribed inP. nordicum under ochratoxin A producing conditions, indicating that this gene is involved in ochratoxin A biosynthesis.
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