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Updated: May 12, 2026
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High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities
Published on: November 15, 2013
[Not Available]
F Horn1, G Hanschmann, C Gebhart
1Hochschule Anhalt, FB Landwirtschaft, Ökotrophologie, Landespflege, Strenzfelder Allee 28, 06406, Bernburg.
Mycotoxin Research
|April 23, 2013
Summary
This study analyzed 98 wheat samples for Fusarium species and mycotoxins deoxynivalenol (DON), zearalenone (ZEA), and nivalenol (NIV). DON analysis showed good correlation between ELISA and HPLC-MS methods, with ELISA yielding higher values.
Area of Science:
- Agricultural Science
- Mycology
- Food Safety
Context:
- Investigated Fusarium species and mycotoxin contamination in wheat.
- Focused on samples from Saxony, harvested in 1998.
- Examined deoxynivalenol (DON), zearalenone (ZEA), and nivalenol (NIV).
Purpose:
- To identify Fusarium species in wheat samples.
- To quantify the levels of DON, ZEA, and NIV in harvested wheat.
- To compare analytical methods for mycotoxin detection.
Summary:
- Analyzed 98 wheat samples using fast-ELISA and HPLC-MS for DON, HPLC-MS for NIV, and HPLC-fluorescence for ZEA.
- Found a strong correlation between ELISA and HPLC-MS for DON detection.
- Observed a trend of higher DON quantification by ELISA compared to HPLC-MS.
Impact:
- Provides insights into mycotoxin contamination in wheat.
- Highlights the reliability and potential biases of different analytical techniques.
- Contributes to understanding Fusarium mycotoxin profiles in agricultural products.
