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Content of mycotoxins in conidiospores from mould in buildings

K Senkpiel1, D Sassenberg, R Keller

  • 1Department of Medical Microbiology and Hygiene, Medical University of Lübeck, Ratzeburger Allee 160, 23538, Lübeck.

Mycotoxin Research
|April 23, 2013
PubMed

Insights

Mechanical disintegration effectively extracts mycotoxins from mold conidiospores. This method allows quantification of various toxins, including patulin and aflatoxins, from indoor mold strains.

Area of Science:

  • Mycology
  • Analytical Chemistry
  • Environmental Science

Background:

  • Indoor mold contamination poses health risks due to mycotoxin production.
  • Accurate quantification of mycotoxins from conidiospores is crucial for risk assessment.

Purpose of the Study:

  • To develop and validate an efficient method for extracting conidiospores from mold cultures.
  • To quantify specific mycotoxins produced by indoor mold strains.

Main Methods:

  • Tested various extraction and disintegration methods for conidiospores.
  • Employed mechanical disintegration using a ball mill with glass granules.
  • Quantified mycotoxins (patulin, gliotoxin, aflatoxins, ochratoxin A, satratoxin H) using analytical techniques.

Main Results:

  • Mechanical disintegration with a ball mill proved most effective for conidiospore processing.
  • Quantified multiple mycotoxins from four toxin-positive wild mold strains.
  • Mycotoxin concentrations ranged from 0.004 µg to 10.3 µg per 10^7 or 10^8 conidiospores.

Conclusions:

  • Mechanical disintegration is a suitable method for isolating conidiospores for mycotoxin analysis.
  • This study provides valuable data on mycotoxin profiles of indoor molds.

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