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Related Experiment Videos

Selenium utilization in humans--a long-term, self-labeling experiment with stable isotopes.

C Veillon1, K Y Patterson, L N Button

  • 1Beltsville Human Nutrition Research Center, US Department of Agriculture, MD 20705.

The American Journal of Clinical Nutrition
|July 1, 1990
PubMed
Summary

Stable selenium isotopes labeled deep body pools, revealing excretion primarily via urine (80%) and enabling accurate nutrient absorption studies by measuring gastrointestinal reexcretion.

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Area of Science:

  • Nutritional Biochemistry
  • Trace Element Metabolism
  • Isotope Tracing

Background:

  • Understanding selenium (Se) bioavailability and turnover is crucial for human health.
  • Conventional balance studies often contain errors due to unmeasured gastrointestinal reexcretion.
  • Deep body pools represent significant Se reservoirs with slow exchange rates.

Purpose of the Study:

  • To label deep body selenium pools using stable isotopes for tracer studies.
  • To investigate the excretion pathways of selenium from these deep pools.
  • To develop a more accurate method for assessing selenium absorption.

Main Methods:

  • Oral administration of stable selenium isotopes (selenomethionine or selenite) for 3 weeks.
  • Reinfusion of labeled blood plasma as a tracer dose after 11 months of storage.

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  • Measurement of selenium distribution in accessible tissues and excretion products (urine, feces).

    • Main Results:

    • Accessible tissues, like red blood cells, showed high labeling (20-25%) after selenomethionine administration.
    • Selenium from deep body pools was predominantly excreted via urine (approximately 80%).
    • Gastrointestinal reexcretion of absorbed selenium was quantifiable, allowing for error correction.

    Conclusions:

    • Stable selenium isotopes effectively label deep body pools for metabolic studies.
    • Urinary excretion is the primary route for eliminating selenium from deep body compartments.
    • Measuring gastrointestinal reexcretion provides a more accurate assessment of selenium absorption compared to conventional methods.