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Fumonisin B1: Isolation from corn culture, and purification by high performance liquid chromatography
R Vesonder1, R Peterson, R Plattner
1Mycotoxin Research, US Department of Agriculture, Agricultural Research Service, Northern Regional Research Center, 1815 North University Street, 61604, Peoria, IL, USA.
Abstract:
A method is described to isolate fumonisin B1 (FB1) from corn cultured for 18 days at 25°C withFusarium moniliforme. Cultured corn was extracted with aqueous methanol and purified with XAD-2 column chromatography and high performance liquid chromatography (HPLC). About 450 mg of FB1 were obtained from 800g cultured corn. Its identity was established by fast-atom bombardment (FAB) mass spectrometry, and infrared spectrum and nuclear magnetic spectrum. Its purity was estimated to be 95% by gas chromatography/mass spectrometry (GC/MS).References.
