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Updated: May 12, 2026
Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
Published on: April 23, 2012
Rapid fluorometric test for the quantitative determination of deoxynivalenol in raw cereals
M Hafner1, Z Kubus, M Freudenschuss
1Christian Doppler Laboratory for Mycotoxin Research Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Applied Life Sciences, Konrad Lorenz Stra\e 20, 3430, Vienna, Tulln, Austria.
Abstract:
Fast test systems fulfilling legislative specifications are required to determine mycotoxin contamination in unprocessed cerealse.g. at grain elevators, import and export terminals, or the milling and brewing industry to prevent contamination of food and feed.We describe the first tests of a novel fluorescence-based test for deoxynivalenol (DON), which will be commercially available within the next few months.The analytical procedure of the new test takes less than 15 minutes for extraction, purification, derivatization and measurement. The system's advantage is its speed and easy procedure providing quantitative DON determination.To ensure an international standard, the validation procedure for wheat, barley and maize will be performed following USDA/GIPSA requirements (03/2006) for DON tests.
