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Related Experiment Video

Updated: May 12, 2026

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
10:01

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays

Published on: April 23, 2012

Airborne mycotoxins in dust from grain elevators.

S Mayer1, V Curtui, E Usleber

  • 1Employers' Liability Insurance Association (Grox03B2;handels- und Lagerei Berufsgenossenschaft, Dept. of prevention, M5, 7, 68161, Mannheim, Germany, S.Mayer@grolabg.de.

Mycotoxin Research
|April 23, 2013
PubMed
Summary

Grain elevator dust contains ochratoxin A, deoxynivalenol, and zearalenone. While airborne mycotoxin exposure is low, unknown cytotoxic compounds were detected, warranting further research into assessment criteria.

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Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA
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Last Updated: May 12, 2026

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Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA
12:02

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA

Published on: May 2, 2018

Area of Science:

  • Environmental Science
  • Occupational Health
  • Toxicology

Background:

  • Grain elevator workers face potential exposure to mycotoxins via grain dust.
  • Mycotoxins like ochratoxin A (OTA), deoxynivalenol (DON), and zearalenone (ZEA) are common contaminants in grains.

Purpose of the Study:

  • To assess the mycotoxin burden in German grain elevators.
  • To evaluate the cytotoxicity of grain dust samples.
  • To estimate airborne mycotoxin concentrations and their occupational health relevance.

Main Methods:

  • Collected 35 settled dust samples from 13 German grain elevators.
  • Analyzed dust for OTA, DON, and ZEA using specific detection limits.
  • Assessed sample cytotoxicity using a MTT bioassay with swine kidney cells.
  • Determined airborne dust concentrations.

Main Results:

  • Nearly all samples contained OTA (96%), DON (100%), and ZEA (100%) with median concentrations of 0.4, 416, and 126 ng/g, respectively.
  • 86% of samples exhibited cytotoxic effects, but not correlated with mycotoxin levels, suggesting unknown toxins.
  • Estimated average airborne concentrations: OTA (0.002 ng/m³), DON (2 ng/m³), ZEA (1 ng/m³).

Conclusions:

  • Inhalatory intake of mycotoxins in grain elevators is likely below tolerable daily intake levels.
  • The presence of unidentified cytotoxic compounds in grain dust requires further investigation.
  • Development of assessment criteria for airborne mycotoxin exposure is crucial for occupational health.