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In german food control laboratories fumonisins mostly are analysed by HPLC with fluorescence detection (HPLC-FLD; with immunoaffinity cleanup) or HPLC-MS/MS (without any cleanup), while cattle feed is often analysed by ELISA for fumonisin FB1. 17 samples of corn flour (11 samples) and corn grit (6 samples) were analysed by the three different methods in order to rate the use of ELISA in public authority food control. Moreover ELISA cross reactions of fumonisins FB2, FB3, hFB1 and hFB2 were determined in order to quantify the influence of these reactions on ELISA results.The comparison showed that HPLC-MS/MS always gave higher contents than HPLC-FLD (20% up to 40%). That only can be explained by losses of fumonisins in the cleanup step of HPLC-FLD. In comparison with that there is a good correlation between HPLC-MS/MS results and ELISA results if the cross reactions are considered.As conclusion the ELISA can be used alternatively as a fast but sometimes even expensive screening method for determination of fumonisins.