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Determination of ochratoxin A by immunoaffinity column clean-up and HPLC In wheat and pig liver
E C Marley1, W C Nicol, A A Candlish
1Rhône Poulenc Diagnostics West of Scotland Science Park 3.06 Kelvin, Campus Maryhill Road, G20 OSP, Glasgow, Scotland.
Abstract:
Determination of ochratoxin A (OTA) by immunoaffinity column clean-up and HPLC detection was performed on wheat and pig liver. Several extraction protocols involving methanol and ethyl acetate were investigated. The optimum experimental conditions for analysis of OTA in artificially contaminated wheat (87.4% recovery) using immunoaffinity column clean-up was found to be the methanol: PBS (1∶1 v/v) protocol. These conditions, however, gave low recoveries for pig liver (40.4%).
