Effects of deoxynivalenol and lipopolysaccharide on electrophysiological parameters in growing pigs

Deoxynivalenol (DON) is a major B-trichothecene that draws importance from its natural occurrence in cereals worldwide. It has many effects on rapidly dividing cells. Lipopolysaccharide (LPS) is an endotoxin released from most Gram-negative bacteria, which plays a major role in induction of inflammation and sepsis under certain conditions. In our experiments we aimed to study the effects of different concentrations of DON (up to 8,000 ng/ml) on the electrogenic transport of nutrients and on tissue conductances in growing pigs using the Ussing chamber technique. The effect of DON-contaminated feed (2.9 mg/kg feed) on the respective parameters, as well as the interactions between DON and intraperitoneal (i.p.) LPS were assessed using porcine jejunal tissues. In vitro DON inhibited the absorption of alanine and glucose across the pig jejunum at concentrations of 4,000 and 8,000 ng/ml, suggesting that DON had an inhibitory effect on the electrogenic transport of nutrients across porcine small intestines. Electrogenic transport of alanine and glucose across porcine small intestines varied regionally among intestinal segments with higher response in ileal tissues. A synergistic effect was observed between DON in feed and injected LPS on tissue conductance. In response, glucose with higher short circuit currents was observed across porcine jejunal mucosa in nutrient stimulated conditions.