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A general synthetic method toward uridylylated picornavirus VPg proteins
Gerbrand J van der Heden van Noort1, Catherine H Schein, Herman S Overkleeft
1Leiden Institute of Chemistry, Leiden University, P. O. Box 9502, RA, 2300, Leiden, The Netherlands.
Abstract:
Small proteins called viral protein genome-linked (VPg), attached to the 5'-end of the viral RNA genome are found as common structure in the large family of picornaviruses. The replication of these viruses is primed by this VPg protein linked to a single uridylyl residue. We report a general procedure to obtain such nucleoproteins employing a pre-uridylylated tyrosine building block in an on-line solid phase-based approach.
