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Immunoreactive LH in long-term frozen human urine samples
Gurmeet Kaur Surindar Singh1, Mark Jimenez, Ron Newman
1Andrology Laboratory, ANZAC Research Institute, Sydney, NSW 2139, Australia; Faculty of Pharmacy, Universiti Teknologi MARA, Bandar Puncak Alam, Selangor, 42300, Malaysia.
Drug Testing and Analysis
|April 23, 2013
Summary
Urinary luteinizing hormone (LH) can be measured using immunoassays. The immunochemiluminometric (ICL) assay provides robust, reproducible results for quantitative analysis, while the immunofluorometric (IF) assay is suitable for within-study comparisons.
Area of Science:
- Endocrinology
- Clinical Chemistry
- Biochemical Analysis
Background:
- Urine offers a non-invasive method for hormone level measurement, contrasting with blood sampling.
- Urinary luteinizing hormone (LH) analysis is valuable in endocrinology research and anti-doping efforts.
- Existing LH immunoassays are validated for blood, necessitating urine-specific validation.
Purpose of the Study:
- To evaluate the reliability of immunofluorometric (IF) and immunochemiluminometric (ICL) LH assays for measuring urinary LH.
- To assess the impact of prolonged frozen storage (-20°C) on the accuracy of urinary LH measurements.
Main Methods:
- Serial urine samples were collected after recombinant human chorionic hormone (rhCG) administration.
- Urinary LH levels were measured using IF and ICL assays in 2008 and again in 2012 after frozen storage.
- Samples were stored at -20°C without preservatives.
Main Results:
- The ICL assay demonstrated quantitatively reproducible urinary LH measurements after four years of frozen storage.
- The IF immunoassay yielded lower LH levels compared to ICL, with a further reduction after storage.
- Both assays showed similar temporal patterns in urinary LH measurements before and after storage.
Conclusions:
- Both IF and ICL immunoassays are suitable for urinary LH measurement.
- The ICL assay is more robust for quantitative applications, such as anti-doping tests.
- The IF assay is appropriate for research studies comparing LH levels within a study, but not for absolute quantification.
