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Updated: May 12, 2026
Isolation of Mouse Endometrial Epithelial and Stromal Cells for In Vitro Decidualization
Published on: March 2, 2017
Epithelial-stromal interactions in the mouse vagina exposed neonatally to diethylstilbestrol
Tomoko Katoh1, Shinji Hayashi, Taisen Iguchi
1Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama, Japan. tomomi@yokohama-cu.ac.jp
Background:
In neonate mice exposed to diethylstilbestrol (neoDES), vaginal epithelium shows persistent proliferation and stratification even after ovariectomy. Tissue recombination studies suggest that neonatally-estrogenized vaginal stroma can induce vaginal epithelial hyperplasia depending on the stromal age. This study examined the proliferative effect of the vaginal stroma from 8-day-old mice treated with DES on the vaginal epithelia of 8-day-old and adult mice.
Materials And Methods:
Vaginal epithelium and stroma from 8-day-old and adult mice was recombined, and grafted to ovariectomized host mice.
Results:
The 5-bromo-2'-deoxyuridine (BrdU)-labeled cells in the epithelium and the number of epithelial cell layers were not significantly different between epithelia from 8-day-old and adult mice when combined with stroma from 8-day-old control mice. BrdU-labeled cells in the vaginal epithelia from both age groups combined with the stroma from 8-day-old neoDES mice exhibited higher values. The epithelium from neoDES adult mice had a lower percentage of BrdU-labeled cells.
Conclusion:
The stroma from 8-day-old neoDES mice induces epithelial cell proliferation, but lower stromal cell proliferation.
