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Updated: May 12, 2026

Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications
13:51

Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications

Published on: November 10, 2017

Multicolor upconversion nanoparticles for protein conjugation.

Stefan Wilhelm1, Thomas Hirsch, Wendy M Patterson

  • 1University of Regensburg, Institute of Analytical Chemistry, Chemo- and Biosensors, 93040 Regensburg, Germany.

Theranostics
|April 23, 2013
PubMed
Summary
This summary is machine-generated.

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We developed lanthanide-doped upconverting nanoparticles for protein conjugation. These nanoparticles can be functionalized for bioconjugation, enabling multicolor imaging applications.

Area of Science:

  • Nanotechnology
  • Bioconjugation
  • Luminescent Materials

Background:

  • Lanthanide-doped upconverting nanoparticles (UCNPs) offer unique optical properties.
  • Functionalization of UCNPs is crucial for their application in biological systems.

Purpose of the Study:

  • To prepare monodisperse, lanthanide-doped hexagonal-phase NaYF4 upconverting luminescent nanoparticles (UCNPs) for protein conjugation.
  • To functionalize these UCNPs with poly(ethylene glycol) (PEG) spacers and N-hydroxysuccinimide (NHS) ester groups for enhanced reactivity and bioconjugation.

Main Methods:

  • Preparation of monodisperse, lanthanide-doped hexagonal-phase NaYF4 UCNPs.
  • Coating UCNPs with a silica shell, followed by modification with PEG spacers and NHS ester groups.
  • Characterization using transmission electron microscopy (TEM), Raman spectroscopy, X-ray diffraction (XRD), and dynamic light scattering (DLS).
Keywords:
Raman spectroscopyUpconversion luminescencebiosensingmagnetic separationnanoparticlesilica coatingsurface plasmon resonance.

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A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles
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A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

Published on: November 14, 2015

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Last Updated: May 12, 2026

Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications
13:51

Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications

Published on: November 10, 2017

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles
12:51

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

Published on: November 14, 2015

  • Conjugation of functionalized UCNPs to proteins (streptavidin and bovine serum albumin).
  • Analysis of bioconjugation using surface plasmon resonance (SPR).

    • Main Results:

    • Successfully synthesized monodisperse, lanthanide-doped hexagonal-phase NaYF4 UCNPs.
    • Achieved silica shell coating and functionalization with PEG spacers and NHS ester groups, rendering particles reactive towards amine nucleophiles.
    • Demonstrated successful conjugation of UCNPs to proteins.
    • Observed multicolor emissions from protein-conjugated UCNPs upon laser excitation.
    • Confirmed bioconjugation and compared particle affinity for immobilized proteins using SPR.

    Conclusions:

    • Developed a method for preparing protein-reactive UCNPs.
    • Demonstrated the utility of these UCNPs for multicolor bioimaging through successful protein conjugation.
    • The functionalized UCNPs show promise for various biosensing and imaging applications.