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Cloning and heterospecific expression of the resistance determinant vanA encoding high-level resistance to
A Brisson-Noël1, S Dutka-Malen, C Molinas
1Unité des Agents Antibactériens, Unité Associée Centre National de la Recherche Scientifique 271, Institut Pasteur, Paris, France.
Antimicrobial Agents and Chemotherapy
|May 1, 1990
Abstract:
Fragments of plasmid pIP816, which confers high-level glycopeptide resistance in Enterococcus faecium BM4147, were cloned into a conjugative gram-negative-gram-positive shuttle vector. The resulting hybrids were transferred by conjugation from Escherichia coli to Enterococcus faecalis and Bacillus thuringiensis. A 4-kilobase EcoRI fragment from pIP816 was found to confer vancomycin resistance in these hosts but not in E. coli or Bacillus subtilis.