Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Adenovirus-delivered angiopoietin-1 suppresses NF-κB and p38 MAPK and attenuates inflammatory responses in phosgene-induced acute lung injury.

Inhalation toxicology·2014
Same author

Exposing to cadmium stress cause profound toxic effect on microbiota of the mice intestinal tract.

PloS one·2014
Same author

Association of KCNQ1 and KLF14 polymorphisms and risk of type 2 diabetes mellitus: A global meta-analysis.

Human immunology·2014
Same author

Treatment of AO/OTA 31-A3 intertrochanteric femoral fractures with a percutaneous compression plate.

Clinics (Sao Paulo, Brazil)·2014
Same author

Plasma metabolomic profiles in breast cancer patients and healthy controls: by race and tumor receptor subtypes.

Translational oncology·2014
Same author

Enzyme-controllable F-NMR turn on through disassembly of peptide-based nanospheres for enzyme detection.

Organic & biomolecular chemistry·2014

Related Experiment Video

Updated: May 12, 2026

Regioselective Biolistic Targeting in Organotypic Brain Slices Using a Modified Gene Gun
06:40

Regioselective Biolistic Targeting in Organotypic Brain Slices Using a Modified Gene Gun

Published on: October 24, 2014

Fatty acid-based strategy for efficient brain targeted gene delivery.

Jie Shen1, Mei Yu, Qinggang Meng

  • 1Key Laboratory o f Smart Drug Delivery, Ministry of Education & PLA Department of Pharmaceutics, School of Pharmacy, Fudan University, Shanghai, 201203, People's Republic of China.

Pharmaceutical Research
|April 24, 2013
PubMed
Summary

Researchers developed a novel fatty acid-based strategy for efficient brain gene delivery. Myristic acid-polyethylenimine conjugates demonstrated enhanced brain targeting and gene transfection, offering a promising approach for neurological therapies.

More Related Videos

Focused Ultrasound Induced Blood-Brain Barrier Opening for Targeting Brain Structures and Evaluating Chemogenetic Neuromodulation
08:37

Focused Ultrasound Induced Blood-Brain Barrier Opening for Targeting Brain Structures and Evaluating Chemogenetic Neuromodulation

Published on: December 22, 2020

A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA
06:53

A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA

Published on: December 26, 2015

Related Experiment Videos

Last Updated: May 12, 2026

Regioselective Biolistic Targeting in Organotypic Brain Slices Using a Modified Gene Gun
06:40

Regioselective Biolistic Targeting in Organotypic Brain Slices Using a Modified Gene Gun

Published on: October 24, 2014

Focused Ultrasound Induced Blood-Brain Barrier Opening for Targeting Brain Structures and Evaluating Chemogenetic Neuromodulation
08:37

Focused Ultrasound Induced Blood-Brain Barrier Opening for Targeting Brain Structures and Evaluating Chemogenetic Neuromodulation

Published on: December 22, 2020

A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA
06:53

A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA

Published on: December 26, 2015

Area of Science:

  • Biotechnology
  • Neuroscience
  • Drug Delivery

Background:

  • Efficient gene delivery to the brain remains a significant challenge.
  • Small molecule-mediated strategies are being explored to overcome the blood-brain barrier.
  • Fatty acids offer potential for targeted delivery due to their lipophilic nature.

Purpose of the Study:

  • To investigate a fatty acid-based strategy for efficient brain-targeted gene delivery.
  • To understand the mechanisms of small molecule-mediated brain gene delivery.
  • To evaluate the efficacy of fatty acid-polyethylenimine conjugates for brain gene delivery.

Main Methods:

  • Conjugation of various fatty acids (FAs) with polyethylenimine (PEI25k).
  • In vivo and ex vivo assessment of brain targeting using a near-infrared fluorescence probe (IR820).
  • Evaluation of DNA nanoparticle uptake and gene transfection efficiency in vitro and in vivo using pEGFP.

Main Results:

  • Fatty acid modification did not impede DNA complexation with PEI25k.
  • Fatty acid-PEI25k conjugates exhibited superior brain targeting compared to unmodified PEI25k.
  • Myristic acid-PEI25k (MC-PEI25k) demonstrated sustained brain distribution, enhanced DNA uptake, and efficient in vitro/in vivo gene transfection, with observable GFP expression in brain regions.

Conclusions:

  • Fatty acid modification of PEI25k is a viable strategy for brain gene delivery.
  • Myristic acid-based conjugates show significant promise for efficient brain gene transfection.
  • This approach offers a potential therapeutic avenue for neurological disorders.