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Related Experiment Video

Updated: May 12, 2026

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Compact surface plasmon-enhanced fluorescence biochip.

Koji Toma1, Milan Vala, Pavel Adam

  • 1AIT - Austrian Institute of Technology GmbH, Muthgasse 11, 1190 Vienna, Austria.

Optics Express
|April 24, 2013
PubMed
Summary
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This study introduces a compact biochip that amplifies fluorescence signals using surface plasmons for enhanced biosensing. This novel approach achieves a low limit of detection, making it suitable for sensitive immunoassay applications.

Area of Science:

  • Nanotechnology
  • Biomedical Engineering
  • Optics

Background:

  • Surface plasmon resonance (SPR) offers high sensitivity for biosensing.
  • Enhancing fluorescence signals in bioassays is crucial for improving detection limits.
  • Existing biochip designs face challenges in signal amplification and cost-effective manufacturing.

Purpose of the Study:

  • To develop a compact biochip utilizing surface plasmon-enhanced fluorescence for improved bioassays.
  • To integrate diffractive optical elements with SPR for efficient signal detection.
  • To demonstrate the biochip's capability in sensitive immunoassay applications.

Main Methods:

  • A compact biochip design incorporating a metallic sensor surface.
  • Utilizing a (reverse) Kretschmann configuration for surface plasmon excitation and fluorescence collection.

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A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
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A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Related Experiment Videos

Last Updated: May 12, 2026

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
09:09

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

  • Embedding diffractive optical elements, including concentric and linear gratings, onto the chip surface.

    • Main Results:

    • Achieved amplification of fluorescence signals through coupling with surface plasmons.
    • Demonstrated increased fluorescence intensity and reduced background noise.
    • Successfully implemented the biochip for a model immunoassay, reaching a limit of detection of 11 pM.

    Conclusions:

    • The developed biochip concept offers a significant advancement in surface plasmon-enhanced fluorescence assays.
    • The integration of diffractive optics and SPR provides efficient signal enhancement and detection.
    • The biochip's compatibility with nanoimprint lithography ensures cost-effective production for sensitive biosensing applications.