Discrimination between antibodies to HIV-1 and HIV-2 in sera from northern Zaire

136 selected sera from Northern Zaire were screened for antibodies against HIV-1 and HIV-2 using the ELISA method. Reactive sera were confirmed with commercially available Western Blots and additional tests. A data base was used for evaluation of the results. The sera were collected in the town Isiro and in remote rural areas. 36 sera were reactive in the HIV-1 ELISA (ELAVIA-1), 19 of them were confirmed in the HIV-1 Western Blot (Biorad). 21 sera were reactive in the HIV-2 ELISA, but they were not confirmed as HIV-2-specific in three HIV-2 Western Blots (Diagnostics Pasteur, Biorad, Paul-Ehrlich-Institut).