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Related Experiment Video

Updated: May 12, 2026

Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software
08:32

Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software

Published on: March 24, 2011

Practical tips for imaging ascidian embryos.

Takefumi Negishi1, Alex McDougall, Hitoyoshi Yasuo

  • 1UPMC Univ Paris 06 and Centre National de la Recherche Scientifique, Laboratoire de Biologie du Développement de Villefranche-sur-Mer, Observatoire Océanologique, 06234, Villefranche-sur-mer, France.

Development, Growth & Differentiation
|April 25, 2013
PubMed
Summary
This summary is machine-generated.

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Researchers have mapped the gene regulatory network for chordate larval development. This study integrates this blueprint with cellular processes using advanced imaging techniques for ascidian embryogenesis.

Area of Science:

  • Developmental biology
  • Molecular biology
  • Genetics

Background:

  • Gene regulatory networks (GRNs) are crucial for development.
  • Previous studies established a GRN for chordate larval tissue types.
  • Integrating GRNs with morphogenetic processes remains a challenge.

Purpose of the Study:

  • To integrate the ascidian gene regulatory network with morphogenetic and cellular processes.
  • To showcase the application of advanced imaging and fluorescent protein technologies in developmental biology.
  • To provide a practical guide for preparing ascidian embryos for imaging.

Main Methods:

  • Utilizing recent advances in imaging technologies.
  • Employing fluorescent protein (FP) techniques.

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Related Experiment Videos

Last Updated: May 12, 2026

Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software
08:32

Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software

Published on: March 24, 2011

Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus
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Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus

Published on: January 12, 2015

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  • Developing protocols for ascidian embryo preparation for microscopy.

    • Main Results:

    • Demonstrated the application of new imaging and FP technologies to ascidian embryogenesis.
    • Provided a detailed practical guide for ascidian embryo preparation.
    • Laid groundwork for integrating genetic and physical processes in development.

    Conclusions:

    • Advanced imaging and FP technologies are powerful tools for studying complex developmental processes.
    • Integrating gene regulatory networks with cellular and morphogenetic events is essential for a complete understanding of embryogenesis.
    • This work facilitates future research in ascidian developmental biology and chordate evolution.