Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Translational Regulation01:29

Translational Regulation

Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
Prokaryotic Gene Structure and Organization01:28

Prokaryotic Gene Structure and Organization

Prokaryotic genomes exhibit a streamlined organization of coding and non-coding regions essential for gene expression and protein synthesis. While coding regions contain the genetic instructions for proteins or functional RNAs, non-coding regions regulate the precise transcription and translation of these genes.Coding Regions: Proteins and RNAsThe primary coding regions, known as structural genes, include sequences transcribed into messenger RNA (mRNA) and ultimately translated into...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Translation Inhibiting Antibiotics Induce an asRNA Regulating Purine Metabolism in <i>S. pneumoniae</i> TIGR4 via an ISL3 Insertion Derived Hybrid Promoter.

bioRxiv : the preprint server for biology·2026
Same author

How Binding Affinity and Binding Specificity Map to Sequence Space.

Journal of molecular evolution·2026
Same author

Electrical readout strategies of GFET biosensors for real-world requirements.

Biosensors & bioelectronics·2026
Same author

Analysis of 3'-seq data from multiple <i>E. coli</i> studies identifies diverging results sets and raw data characteristics despite similar collection conditions.

bioRxiv : the preprint server for biology·2025
Same author

Assessing the conservation and targets of putative sRNAs in <i>Streptococcus pneumoniae</i>.

Microbiology spectrum·2025
Same author

Evaluation of novel computational methods to identify RNA-binding protein footprints from structural data.

RNA (New York, N.Y.)·2025

Related Experiment Video

Updated: May 12, 2026

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses
11:19

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses

Published on: February 25, 2011

RNA structures regulating ribosomal protein biosynthesis in bacilli.

Kaila Deiorio-Haggar1, Jon Anthony, Michelle M Meyer

  • 1Boston College Department of Biology; Chestnut Hill, MA USA.

RNA Biology
|April 25, 2013
PubMed
Summary

Three bacterial regulatory RNAs in Bacilli, controlling ribosomal protein synthesis, show unique structures and limited phylogenetic distribution within the Bacilli class. Further discoveries of similar RNA elements are expected in other bacterial species.

Keywords:
Bacillus subtilisGeobacillus stearothermophilusInfernalRfamgram-positiveribosomal leader sequenceribosomal protein

More Related Videos

Peering at Brain Polysomes with Atomic Force Microscopy
08:49

Peering at Brain Polysomes with Atomic Force Microscopy

Published on: March 16, 2016

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
12:05

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

Published on: August 7, 2021

Related Experiment Videos

Last Updated: May 12, 2026

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses
11:19

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses

Published on: February 25, 2011

Peering at Brain Polysomes with Atomic Force Microscopy
08:49

Peering at Brain Polysomes with Atomic Force Microscopy

Published on: March 16, 2016

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
12:05

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

Published on: August 7, 2021

Area of Science:

  • Bacterial genomics and transcriptomics
  • Molecular biology and RNA structure
  • Evolutionary biology of regulatory elements

Background:

  • Ribosomal protein autogenous regulation is crucial for bacterial growth.
  • Specific regulatory RNAs in Bacilli (S4, S15, L20) are not found in E. coli.
  • Existing databases lack comprehensive information on these Bacilli-specific RNAs.

Purpose of the Study:

  • To investigate the phylogenetic distribution of three Bacilli-specific ribosomal-protein regulatory RNAs.
  • To characterize the secondary structures and evolutionary conservation of these RNAs.
  • To identify potential novel RNA regulatory elements in other bacterial species.

Main Methods:

  • Creation and modification of structural alignments for the three target RNAs.
  • Homology searches using the curated structural alignments.
  • Phylogenetic analysis to determine the distribution of identified RNA structures.

Main Results:

  • The three regulatory RNAs (interacting with ribosomal proteins S4, S15, and L20) exhibit a narrow phylogenetic distribution.
  • These RNAs are predominantly found within the Firmicute class, specifically in Bacilli.
  • Only the L20-interacting RNA was previously documented in the RNA Families Database.

Conclusions:

  • The characterized regulatory RNAs are specific to the Bacilli class, indicating specialized gene regulation.
  • The findings suggest a broader, yet undiscovered, landscape of non-homologous RNA regulatory elements across bacterial species.
  • Further research is needed to discover and characterize these novel RNA regulatory mechanisms in diverse bacterial taxa.