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Point-of-care CRISPR-based Diagnostics with Premixed and Freeze-dried Reagents

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A novel handheld fluorescent microarray reader for point-of-care diagnostic.

P Kozma¹, A Lehmann, K Wunderlich

¹Fraunhofer Institute for Biomedical Engineering (IBMT), Am Mühlenberg 13, 14476 Potsdam-Golm, Germany. peter.kozma@ibmt.fraunhofer.de

Biosensors & Bioelectronics

|April 25, 2013

Summary

This summary is machine-generated.

A new handheld optical sensor, portMD-113, quantifies fluorescent microarrays. It offers comparable detection limits to commercial readers but is compact, low-cost, and battery-powered for point-of-care diagnostics.

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Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

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Published on: October 1, 2016

Area of Science:

Biomedical Engineering
Optical Sensing Technology
Microarray Analysis

Background:

Fluorescent microarrays are crucial for biological complex quantification.
Existing microarray readers are often bulky, expensive, and energy-intensive.
There is a need for portable, cost-effective microarray quantification solutions.

Purpose of the Study:

To develop a novel handheld optical sensor for fluorescent microarray quantification.
To demonstrate the sensor's performance and advantages over existing technologies.
To explore its potential for point-of-care medical applications.

Main Methods:

Development of the portMD-113 handheld optical sensor.
Utilizing a planar waveguide and evanescent field for fluorescent excitation.
Employing a pinhole array, microlens array, interference filter, and detector array for signal detection.

Main Results:

The portMD-113 achieves detection limits comparable to commercial laser scanners.
The device is compact, lightweight, and low-cost, featuring no moving parts.
It can be powered by a battery or personal computer, enabling portability.

Conclusions:

The portMD-113 offers a cost-effective and portable solution for fluorescent microarray quantification.
Its design advantages make it suitable for point-of-care medical diagnostics.
This technology has the potential to broaden the accessibility of microarray analysis.