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Updated: May 12, 2026

Cultivation Methods of Spirochetes from Borrelia burgdorferi Sensu Lato Complex and Relapsing Fever Borrelia
Published on: November 25, 2022
The growth, lipid and hydrocarbon production of Botryococcus braunii with attached cultivation
Pengfei Cheng1, Bei Ji, Lili Gao
1Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, PR China.
Abstract:
The green alga Botryococcus braunii is regarded as a potential source of renewable fuel due to its high lipid and hydrocarbon contents. However, the slow growth rate damaged its feasibility for biofuel production. In this study, a novel method of 'attached cultivation' was introduced to incubate B. braunii FACHB 357 (B race). A high biomass productivity of 6.5 gm(-2)d(-1) was achieved in single layer attached system at early stage of cultivation. At day 10, the biomass, lipid and hydrocarbon productivities were 5.5, 2.34 and 1.06 gm(-2)d(-1), respectively. Under nitrogen starvation condition, both of the contents of lipid and hydrocarbon were increased, whereas the profile of hydrocarbon kept almost unchanged, while the content for oleic acid (18:1) increased and linolenic acid (18:3) decreased. With a multi-layer photobioreactor, a biomass productivity of 49.1 gm(-2)d(-1) or a photosynthetic efficiency of 14.9% (visible light) were obtained under continuous illumination of 500 μmolm(-2)s(-1).
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