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Quantification of vancomycin in human serum by LC-MS/MS
Clinical Chemistry and Laboratory Medicine
|April 25, 2013
Summary
A new LC-MS/MS method accurately quantifies vancomycin in serum, crucial for standardizing therapeutic drug monitoring. This reliable assay uses a novel internal standard for enhanced analytical precision in clinical settings.
Area of Science:
- Analytical Chemistry
- Clinical Chemistry
Background:
- Standardizing vancomycin therapeutic drug monitoring (TDM) requires reliable quantification methods.
- Existing routine assays need harmonization for consistent vancomycin measurement in serum.
Purpose of the Study:
- Develop and validate a robust liquid chromatography-tandem mass spectrometry (LC-MS/MS) procedure.
- Quantify vancomycin in human serum for TDM standardization.
Main Methods:
- Serum samples underwent protein precipitation and ultrafiltration.
- Extended HPLC separation (9.8 min retention time) minimized matrix effects.
- LC-MS/MS measurement utilized a chemically synthesized vancomycin-glycin internal standard (ISTD) for coelution.
Main Results:
- Bi-center validation showed total coefficients of variation (CVs) <4% for quality control materials (5.3–79.4 mg/L).
- Accuracy was within ±4%, with no significant ion suppression observed.
- Comparative analysis of 70 samples across two sites demonstrated excellent agreement.
Conclusions:
- The developed LC-MS/MS method offers high analytical reliability for serum vancomycin quantification.
- Utilizing a closely related homologue as an internal standard and efficient HPLC pre-fractionation enhances accuracy.
- This validated method is suitable for a comprehensive reference measurement system for vancomycin.
